Datasheet

生物学的記述

Specificity	PDE4B Antibody [A23H17] detects endogenous levels of total PDE4B protein.
Background	PDE4B is a cAMP‑specific phosphodiesterase of the PDE4 family that hydrolyzes 3′,5′‑cAMP to AMP and sets local cAMP levels in multiple immune and neural cell types, placing it as a key negative regulator of cAMP‑dependent signaling. The protein contains the conserved catalytic core of class I PDEs, with metal‑binding His and Asp residues forming the active site that coordinates divalent cations and positions cAMP for nucleophilic attack, and N‑terminal regulatory regions that target PDE4B isoforms to defined subcellular compartments and scaffold complexes. In dendritic cells and monocytes, PDE4B is a predominant cAMP‑degrading enzyme, and its activity limits cAMP accumulation after receptor stimulation, thereby controlling the amplitude and duration of PKA‑ and EPAC‑dependent phosphorylation events that influence NF‑κB, CREB, and other transcription factors. By shaping these cAMP gradients, PDE4B regulates production of inflammatory cytokines such as TNF‑α, IL‑6, and IL‑1β, affects expression of costimulatory molecules, and modulates the balance between pro‑ and anti‑inflammatory programs during responses to bacterial lipopolysaccharide and other stimuli. In cardiac myocytes, PDE4B localizes to a sarcolemmal compartment associated with β1‑adrenergic receptors, and its catalytic activity forms part of a local negative feedback loop that constrains cAMP signals generated by β1‑adrenergic stimulation, thereby tuning PKA phosphorylation of nearby substrates that regulate calcium handling and contractility. Genetic deletion or pharmacologic inhibition of PDE4B in this compartment leads to enhanced β1‑adrenergic cAMP responses and altered phosphorylation of proteins linked to excitation–contraction coupling.

Specificity

PDE4B Antibody [A23H17] detects endogenous levels of total PDE4B protein.

Background

PDE4B is a cAMP‑specific phosphodiesterase of the PDE4 family that hydrolyzes 3′,5′‑cAMP to AMP and sets local cAMP levels in multiple immune and neural cell types, placing it as a key negative regulator of cAMP‑dependent signaling. The protein contains the conserved catalytic core of class I PDEs, with metal‑binding His and Asp residues forming the active site that coordinates divalent cations and positions cAMP for nucleophilic attack, and N‑terminal regulatory regions that target PDE4B isoforms to defined subcellular compartments and scaffold complexes. In dendritic cells and monocytes, PDE4B is a predominant cAMP‑degrading enzyme, and its activity limits cAMP accumulation after receptor stimulation, thereby controlling the amplitude and duration of PKA‑ and EPAC‑dependent phosphorylation events that influence NF‑κB, CREB, and other transcription factors. By shaping these cAMP gradients, PDE4B regulates production of inflammatory cytokines such as TNF‑α, IL‑6, and IL‑1β, affects expression of costimulatory molecules, and modulates the balance between pro‑ and anti‑inflammatory programs during responses to bacterial lipopolysaccharide and other stimuli. In cardiac myocytes, PDE4B localizes to a sarcolemmal compartment associated with β1‑adrenergic receptors, and its catalytic activity forms part of a local negative feedback loop that constrains cAMP signals generated by β1‑adrenergic stimulation, thereby tuning PKA phosphorylation of nearby substrates that regulate calcium handling and contractility. Genetic deletion or pharmacologic inhibition of PDE4B in this compartment leads to enhanced β1‑adrenergic cAMP responses and altered phosphorylation of proteins linked to excitation–contraction coupling.

使用情報

Application

WB, IP

Dilution

WB	IP
1:1000	1:150

Reactivity

Human, Mouse, Rat

Source

Rabbit Monoclonal Antibody

MW

90 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/35387344/
https://pubmed.ncbi.nlm.nih.gov/36278172/

PDE4B Antibody [A23H17]

生物学的記述

使用情報

References

Application Data