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Synonyms | LY231514 | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C20H21N5O6 |
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分子量 | 427.41 | CAS No. | 137281-23-3 | |
Solubility (25°C)* | 体外 | DMSO | 85 mg/mL (198.87 mM) | |
Water | Insoluble | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Pemetrexed (LY231514) is a novel antifolate and antimetabolite for thymidylate synthase (TS), dihydrofolate reductase (DHFR) and glycinamide ribonucleotide formyltransferase (GARFT) with Ki of 1.3 nM, 7.2 nM and 65 nM in cell-free assays, respectively. Pemetrexed induces autophagy and apoptosis. |
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in vitro | Osimertinib+pemetrexed treatment delays the emergence of resistance relative to monotherapy in vitro, also down-regulates the expression of the anti-apoptotic gene PLK1 in PC-9 and H1975.[4] |
in vivo | The combination of omepetinib and pemetrexed enhances apoptosis via a PLK1 signaling pathway, and the anti-tumor effect against xenografts harboring the EGFR mutation in vivo.[4] |
細胞アッセイ | 細胞株 | PC-9 and NCI-H1975 (H1975) cells |
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濃度 | 0.001, 0.01, 0.1, 1, 10 μM | |
反応時間 | 72 h | |
実験の流れ | Cells are plated in 96-well tissue culture plates at 5,000 cells/well and incubated at 37 ℃ for 24 h. Cells are then incubated with titrated concentrations of the drugs (0.001, 0.01, 0.1, 1, 10 μM) or vehicle (DMSO) at 37 ℃ for 72 h. Cell numbers are estimated using the Counting Kit-8. Protein extraction and western blotting are performed. Apoptosis assays are performed using the Annexin V-FITC Apoptosis Detection Kit. |
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動物実験 | 動物モデル | Female severe combined immunodeficient (SCID)-Beige mice injected with PC-9 cells |
投薬量 | 100 mg/kg | |
投与方法 | i.p. |
Metabolic Hallmarks for Purine Nucleotide Biosynthesis in Small Cell Lung Carcinoma [ Mol Cancer Res, 2024, 22(1):82-93] | PubMed: 37773022 |
A novel lncRNA-hidden polypeptide regulates malignant phenotypes and pemetrexed sensitivity in A549 pulmonary adenocarcinoma cells [ Amino Acids, 2024, 56(1):15] | PubMed: 38351332 |
Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cells [ Cell Death Dis, 2023, 10.1038/s41419-023-06240-x] | PubMed: 37938546 |
Exploring the Molecular Players behind the Potentiation of Chemotherapy Effects by Durvalumab in Lung Adenocarcinoma Cell Lines [ Pharmaceutics, 2023, 15(5)1485] | PubMed: 37242727 |
Exploring the Molecular Players behind the Potentiation of Chemotherapy Effects by Durvalumab in Lung Adenocarcinoma Cell Lines [ Pharmaceutics, 2023, 15(5)1485] | PubMed: 37242727 |
Gastrodin destabilizes survivin and overcomes pemetrexed resistance [ Cell Signal, 2023, 110:110851] | PubMed: 37586466 |
SETD4 Confers Cancer Stem Cell Chemoresistance in Nonsmall Cell Lung Cancer Patients via the Epigenetic Regulation of Cellular Quiescence [ Stem Cells Int, 2023, 2023:7367854] | PubMed: 37274024 |
Immunofluorescence-Based Assay for High-Throughput Analysis of Multidrug Resistance Markers in Non-Small Cell Lung Carcinoma Patient-Derived Cells [ Diagnostics (Basel), 2023, 13(24)3617] | PubMed: 38132201 |
Immune checkpoint inhibitors modulate the cytotoxic effect of chemotherapy in lung adenocarcinoma cells [ Oncology Letters, 2023, 152] | PubMed: None |
Immune checkpoint inhibitors modulate the cytotoxic effect of chemotherapy in lung adenocarcinoma cells [ Oncol Lett, 2023, 25(4):152] | PubMed: 36936028 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。