Pentylenetetrazol

製品コードS4587 バッチS458701

印刷

化学情報

Synonyms

Pentylenetetrazole, Metrazole, pentetrazol, pentamethylenetetrazol, Corazol, Cardiazol, PTZ

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₆H₁₀N₄

分子量

138.17

CAS No.

54-95-5

Solubility (25°C)*

体外

DMSO

27 mg/mL (195.41 mM)

Water

27 mg/mL (195.41 mM)

Ethanol

27 mg/mL (195.41 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Pentylenetetrazol (Pentylenetetrazole, Metrazole, pentetrazol, pentamethylenetetrazol, Corazol, Cardiazol, PTZ) displays activity as a central nervous system and respiratory stimulant. It is considered a non-competitive GABA(A) receptor antagonist.Pentylenetetrazol can be used to induce animal models of (Acute or Chronic) Epilepsy.
in vivo	Administration of PTZ (45 mg/kg i.p.) induces clonic convulsions in almost all of the animals after a single injection, typically within 3 min. However, 11% of animals need a second injection to cause clonus. Each rat shows jerking or myoclonus followed by clonus or tonic-clonic convulsions. This compound injection results in significant changes in GABAA receptor subunit mRNAs. A single PTZ-induced convulsion is associated with rapid changes in GABAA receptors.

製品説明

Pentylenetetrazol (Pentylenetetrazole, Metrazole, pentetrazol, pentamethylenetetrazol, Corazol, Cardiazol, PTZ) displays activity as a central nervous system and respiratory stimulant. It is considered a non-competitive GABA(A) receptor antagonist.Pentylenetetrazol can be used to induce animal models of (Acute or Chronic) Epilepsy.

in vivo

Administration of PTZ (45 mg/kg i.p.) induces clonic convulsions in almost all of the animals after a single injection, typically within 3 min. However, 11% of animals need a second injection to cause clonus. Each rat shows jerking or myoclonus followed by clonus or tonic-clonic convulsions. This compound injection results in significant changes in GABAA receptor subunit mRNAs. A single PTZ-induced convulsion is associated with rapid changes in GABAA receptors.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	primary cortical neurons isolated from E16 mice embryos
	濃度	0, 7.5, 15, 30, and 60 mM
	反応時間	24 h
	実験の流れ	Primary cortical neurons are obtained from E16 mice embryos and planted in poly-l-lysine and Laminin coated culturing ware in neurobasal medium with 2% B-27 supplement, 0.5% GlutaMAX-ITM supplement, and 1% penicillin-streptomycin. The culture medium is half replaced every 3 days. The primary cells are cultured for 7 days in vitro (DIV) prior to treating them with isometric fresh medium, this compound (30 mM), and this compound (30 mM) + OtoN of different dosages.
動物実験	動物モデル	Male Sprague-Dawley rats
	投薬量	45 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/10336561/
https://pubmed.ncbi.nlm.nih.gov/27504096/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

MicroRNA-9 promotes axon regeneration of mauthner-cell in zebrafish via her6/ calcium activity pathway [ Cell Mol Life Sci, 2024, 81(1):104]	PubMed: 38411738

MicroRNA-9 promotes axon regeneration of mauthner-cell in zebrafish via her6/ calcium activity pathway [ Cell Mol Life Sci, 2024, 81(1):104]

PubMed: 38411738

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。