Datasheet

生物学的記述

Specificity	Phospho-EGFR (Tyr992) Antibody [F20E14] detects endogenous levels of total EGFR protein only when it is phosphorylated at Tyr992.
Background	Phospho-EGFR (Tyr992) is a phosphorylation site on the Epidermal Growth Factor Receptor (EGFR), a transmembrane tyrosine kinase of the HER/ErbB family that regulates cell growth and survival. Tyr992 resides in the intracellular domain near the plasma membrane and, upon phosphorylation, serves as a high-affinity docking site for the SH2 domain of phospholipase C-gamma (PLCγ), leading to PLCγ activation. Activated PLCγ hydrolyzes PIP2 into diacylglycerol (DAG) and inositol trisphosphate (IP3), which trigger protein kinase C (PKC) activation and intracellular calcium release, respectively, promoting cell proliferation and differentiation. This phosphorylation typically occurs after EGF-induced EGFR dimerization. In contrast to other phosphorylation sites like Tyr1045 (recruiting c-Cbl for receptor ubiquitination and degradation) and Tyr1068 (binding Grb2 to activate Ras/MAPK signaling), Tyr992-mediated PLCγ signaling represents a distinct pathway. EGFR phosphorylation thus orchestrates diverse downstream effects through site-specific adaptor protein interactions, regulating processes critical in normal physiology and cancer pathogenesis.

Specificity

Phospho-EGFR (Tyr992) Antibody [F20E14] detects endogenous levels of total EGFR protein only when it is phosphorylated at Tyr992.

Background

Phospho-EGFR (Tyr992) is a phosphorylation site on the Epidermal Growth Factor Receptor (EGFR), a transmembrane tyrosine kinase of the HER/ErbB family that regulates cell growth and survival. Tyr992 resides in the intracellular domain near the plasma membrane and, upon phosphorylation, serves as a high-affinity docking site for the SH2 domain of phospholipase C-gamma (PLCγ), leading to PLCγ activation. Activated PLCγ hydrolyzes PIP2 into diacylglycerol (DAG) and inositol trisphosphate (IP3), which trigger protein kinase C (PKC) activation and intracellular calcium release, respectively, promoting cell proliferation and differentiation. This phosphorylation typically occurs after EGF-induced EGFR dimerization. In contrast to other phosphorylation sites like Tyr1045 (recruiting c-Cbl for receptor ubiquitination and degradation) and Tyr1068 (binding Grb2 to activate Ras/MAPK signaling), Tyr992-mediated PLCγ signaling represents a distinct pathway. EGFR phosphorylation thus orchestrates diverse downstream effects through site-specific adaptor protein interactions, regulating processes critical in normal physiology and cancer pathogenesis.

使用情報

Application

WB, IP, FCM

Dilution

WB	FCM
1:2000	1:500-1:2000

Reactivity

Human

Source

Mouse Monoclonal Antibody

MW

134 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/10069801/
https://pubmed.ncbi.nlm.nih.gov/1537335/

Application Data

WB

Validated by Selleck

Lane 1: A431, Lane 2: A431 (EGF, 100 ng/mL, 37℃, 30 min)