Datasheet

生物学的記述

Specificity	Phospho-HER4/ErbB4 (Tyr1284)/EGFR (Tyr1173) Antibody (Rabbit mAb) [D10B7] detects endogenous levels of HER4/ErbB4 protein only when phosphorylated on Tyr1284 and EGFR only when phosphorylated on Tyr1173.
Background	Phosphorylated HER4/ErbB4 at Tyr1284 and phosphorylated EGFR at Tyr1173 are activation‑dependent epitopes within the cytoplasmic tails of two HER/ErbB family receptor tyrosine kinases, marking ligand‑induced signaling states that integrate extracellular growth factor cues into intracellular cascades controlling proliferation, survival, and differentiation. Both receptors share a modular architecture with an extracellular ligand‑binding domain, a single transmembrane segment, an intracellular tyrosine kinase domain, and a C‑terminal tail containing multiple tyrosine residues that become autophosphorylated upon receptor dimerization, and Tyr1173 in EGFR and Tyr1284 in ErbB4 reside within this tail as key sites for docking of downstream effectors. Upon binding EGF‑family ligands, EGFR transitions from a monomer to active homo‑ or heterodimers (for example with HER2), which stimulates its intrinsic kinase activity and leads to autophosphorylation of several C‑terminal tyrosines, including Tyr1173; the phosphorylated tail recruits adaptor proteins such as Shc and Grb2 that contain SH2 or PTB domains, thereby initiating major downstream pathways including Ras–Raf–MEK–ERK, PI3K–AKT, PLCγ–PKC, and STAT signaling modules that collectively modulate cell migration, adhesion, proliferation, and survival. Phosphorylation at Tyr1173 functions as a prominent docking site for Shc and other adaptors, coordinating assembly of signaling complexes at the receptor and supporting propagation of mitogenic signals, while endocytosis and dephosphorylation by phosphatases provide feedback that attenuates signaling and reset receptor responsiveness. HER4/ErbB4, activated by neuregulins and several EGF‑family ligands, forms homo‑ and heterodimers with other ErbB members, and ligand binding triggers autophosphorylation at multiple tyrosines including Tyr1284, which serve as binding sites for scaffold proteins and effectors that connect the receptor to MAPK and PI3K–AKT pathways, reorganization of the actin cytoskeleton, and cell migration. Specific ErbB4 isoforms containing cytoplasmic sequences compatible with PI3K recruitment use phosphorylated tail tyrosines to bind the p85 regulatory subunit of PI3K, promoting AKT activation and protection from apoptosis, whereas isoforms lacking these motifs or differing in juxtamembrane processing show reduced coupling to PI3K and instead favor alternative signaling outputs, illustrating how the pattern of tail phosphotyrosines, including Tyr1284, shapes receptor‑specific pathway engagement. Proteolytic processing of ErbB4 can release intracellular domains that translocate to the nucleus or mitochondria, where they interact with transcription factors such as STAT5A at promoters like CSN2 to regulate gene expression or engage mitochondrial pathways that influence apoptosis, and phosphorylation of cytoplasmic tail residues in the full‑length receptor precedes and modulates these cleavage‑dependent functions. In developmental and physiological contexts, ErbB4 signaling through its phosphotyrosine sites is required for cardiac muscle differentiation, central nervous system development, and mammary gland differentiation and lactation, while EGFR signaling through Tyr1173 and other sites contributes to epithelial growth, regeneration, and normal tissue homeostasis. In disease settings, aberrant activation and sustained phosphorylation of EGFR Tyr1173 are implicated in oncogenic signaling and cancer progression, driving excessive mitogenic and survival pathway activity, and altered ErbB4 expression or phosphorylation patterns are associated with multiple carcinomas and neuropsychiatric disorders, where changes in ligand responsiveness, dimerization partners, and tail phosphotyrosine combinations can reprogram transcriptional outputs and cell fate decisions.

Specificity

Phospho-HER4/ErbB4 (Tyr1284)/EGFR (Tyr1173) Antibody (Rabbit mAb) [D10B7] detects endogenous levels of HER4/ErbB4 protein only when phosphorylated on Tyr1284 and EGFR only when phosphorylated on Tyr1173.

Background

Phosphorylated HER4/ErbB4 at Tyr1284 and phosphorylated EGFR at Tyr1173 are activation‑dependent epitopes within the cytoplasmic tails of two HER/ErbB family receptor tyrosine kinases, marking ligand‑induced signaling states that integrate extracellular growth factor cues into intracellular cascades controlling proliferation, survival, and differentiation. Both receptors share a modular architecture with an extracellular ligand‑binding domain, a single transmembrane segment, an intracellular tyrosine kinase domain, and a C‑terminal tail containing multiple tyrosine residues that become autophosphorylated upon receptor dimerization, and Tyr1173 in EGFR and Tyr1284 in ErbB4 reside within this tail as key sites for docking of downstream effectors. Upon binding EGF‑family ligands, EGFR transitions from a monomer to active homo‑ or heterodimers (for example with HER2), which stimulates its intrinsic kinase activity and leads to autophosphorylation of several C‑terminal tyrosines, including Tyr1173; the phosphorylated tail recruits adaptor proteins such as Shc and Grb2 that contain SH2 or PTB domains, thereby initiating major downstream pathways including Ras–Raf–MEK–ERK, PI3K–AKT, PLCγ–PKC, and STAT signaling modules that collectively modulate cell migration, adhesion, proliferation, and survival. Phosphorylation at Tyr1173 functions as a prominent docking site for Shc and other adaptors, coordinating assembly of signaling complexes at the receptor and supporting propagation of mitogenic signals, while endocytosis and dephosphorylation by phosphatases provide feedback that attenuates signaling and reset receptor responsiveness. HER4/ErbB4, activated by neuregulins and several EGF‑family ligands, forms homo‑ and heterodimers with other ErbB members, and ligand binding triggers autophosphorylation at multiple tyrosines including Tyr1284, which serve as binding sites for scaffold proteins and effectors that connect the receptor to MAPK and PI3K–AKT pathways, reorganization of the actin cytoskeleton, and cell migration. Specific ErbB4 isoforms containing cytoplasmic sequences compatible with PI3K recruitment use phosphorylated tail tyrosines to bind the p85 regulatory subunit of PI3K, promoting AKT activation and protection from apoptosis, whereas isoforms lacking these motifs or differing in juxtamembrane processing show reduced coupling to PI3K and instead favor alternative signaling outputs, illustrating how the pattern of tail phosphotyrosines, including Tyr1284, shapes receptor‑specific pathway engagement. Proteolytic processing of ErbB4 can release intracellular domains that translocate to the nucleus or mitochondria, where they interact with transcription factors such as STAT5A at promoters like CSN2 to regulate gene expression or engage mitochondrial pathways that influence apoptosis, and phosphorylation of cytoplasmic tail residues in the full‑length receptor precedes and modulates these cleavage‑dependent functions. In developmental and physiological contexts, ErbB4 signaling through its phosphotyrosine sites is required for cardiac muscle differentiation, central nervous system development, and mammary gland differentiation and lactation, while EGFR signaling through Tyr1173 and other sites contributes to epithelial growth, regeneration, and normal tissue homeostasis. In disease settings, aberrant activation and sustained phosphorylation of EGFR Tyr1173 are implicated in oncogenic signaling and cancer progression, driving excessive mitogenic and survival pathway activity, and altered ErbB4 expression or phosphorylation patterns are associated with multiple carcinomas and neuropsychiatric disorders, where changes in ligand responsiveness, dimerization partners, and tail phosphotyrosine combinations can reprogram transcriptional outputs and cell fate decisions.

使用情報

Application

WB

Dilution

WB

1:1000

Reactivity

Human

Source

Rabbit Monoclonal Antibody

MW

147 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/9733788/

Phospho-HER4/ErbB4 (Tyr1284)/EGFR (Tyr1173) Antibody (Rabbit mAb) [D10B7]

生物学的記述

使用情報

References

Application Data