Datasheet

生物学的記述

Specificity	Phospho-MCM2 (Ser139) Antibody [D19G1] detects endogenous levels of total MCM2 protein only when it is phosphorylated at Ser139.
Background	Phospho‑MCM2 (Ser139) marks a key regulatory site on the MCM2 subunit of the MCM2‑7 replicative helicase, which licenses origins and drives DNA‑fork unwinding during S phase. MCM2 is part of a heterohexameric ring that assembles with MCM3–7 at origins as part of the pre‑replication complex, and phosphorylation of MCM2 at Ser139 by the Cdc7/Dbf4 kinase in late G1 and early S phase coincides with the activation of replication forks, linking origin firing to cell‑cycle progression. In mammalian cells, Cdc7‑dependent phosphorylation of Ser139, along with neighboring Ser27 and Ser41, enhances the ATPase activity of the MCM2‑7 ring, promotes chromatin‑bound MCM2 phosphorylation, and is required for the orderly initiation of DNA synthesis, while non‑phosphorylatable MCM2 variants impair replication initiation and reduce helicase efficiency. The MCM2‑7 complex additionally participates in replication‑fork stability and checkpoint responses by interacting with checkpoint and homologous‑recombination regulators, and its dynamic phosphorylation ensures that each origin fires only once per cycle and that stalled forks can be protected and restarted.

Specificity

Phospho-MCM2 (Ser139) Antibody [D19G1] detects endogenous levels of total MCM2 protein only when it is phosphorylated at Ser139.

Background

Phospho‑MCM2 (Ser139) marks a key regulatory site on the MCM2 subunit of the MCM2‑7 replicative helicase, which licenses origins and drives DNA‑fork unwinding during S phase. MCM2 is part of a heterohexameric ring that assembles with MCM3–7 at origins as part of the pre‑replication complex, and phosphorylation of MCM2 at Ser139 by the Cdc7/Dbf4 kinase in late G1 and early S phase coincides with the activation of replication forks, linking origin firing to cell‑cycle progression. In mammalian cells, Cdc7‑dependent phosphorylation of Ser139, along with neighboring Ser27 and Ser41, enhances the ATPase activity of the MCM2‑7 ring, promotes chromatin‑bound MCM2 phosphorylation, and is required for the orderly initiation of DNA synthesis, while non‑phosphorylatable MCM2 variants impair replication initiation and reduce helicase efficiency. The MCM2‑7 complex additionally participates in replication‑fork stability and checkpoint responses by interacting with checkpoint and homologous‑recombination regulators, and its dynamic phosphorylation ensures that each origin fires only once per cycle and that stalled forks can be protected and restarted.

使用情報

Application

WB, IP, IF, FCM

Dilution

WB	IP	IF	FCM
1:1000	1:50	1:100	1:200

Reactivity

Human, Mouse, Rat, Hamster, Monkey, Dog

Source

Rabbit Monoclonal Antibody

MW

125 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/18180284/
https://pubmed.ncbi.nlm.nih.gov/16899510/

Application Data

WB

Validated by Selleck

Lane 1: Hela, Lane 2: Hela (λ phosphatase and CIP treated)