Datasheet

生物学的記述

Specificity	Phospho-STAT1 (Ser727) Antibody [F23C16] detects endogenous levels of total STAT1 protein only when it is Phosphorylated at Ser727.
Background	Phospho-STAT1 (Ser727) is the serine-phosphorylated form of STAT1 at residue Ser727, a modification that plays a pivotal role in regulating STAT1’s transcriptional activity within interferon (IFN) signaling. STAT1, a latent transcription factor, contains several domains: an N-terminal oligomerization domain, a coiled-coil domain, a DNA-binding domain, an SH2 domain for dimerization via Tyr701 phosphorylation, and a C-terminal transactivation domain (TAD) harboring the Ser727 phosphorylation motif (PMSP), which is targeted by MAPKs such as p38. Phosphorylation at Ser727 occurs downstream of IFN-α/γ, cellular stress, or LPS, dependent on prior Tyr701 activation, and enhances STAT1’s interaction with coactivators like CBP/p300. This modification is essential for maximal transcriptional activation of IFN-responsive genes: it augments promoter recruitment and chromatin binding, supporting antiviral responses, macrophage activation, and inflammation. Aberrant Ser727 phosphorylation is linked to tumorigenesis and impaired IFN signaling by disrupting normal STAT1 activity.

Specificity

Phospho-STAT1 (Ser727) Antibody [F23C16] detects endogenous levels of total STAT1 protein only when it is Phosphorylated at Ser727.

Background

Phospho-STAT1 (Ser727) is the serine-phosphorylated form of STAT1 at residue Ser727, a modification that plays a pivotal role in regulating STAT1’s transcriptional activity within interferon (IFN) signaling. STAT1, a latent transcription factor, contains several domains: an N-terminal oligomerization domain, a coiled-coil domain, a DNA-binding domain, an SH2 domain for dimerization via Tyr701 phosphorylation, and a C-terminal transactivation domain (TAD) harboring the Ser727 phosphorylation motif (PMSP), which is targeted by MAPKs such as p38. Phosphorylation at Ser727 occurs downstream of IFN-α/γ, cellular stress, or LPS, dependent on prior Tyr701 activation, and enhances STAT1’s interaction with coactivators like CBP/p300. This modification is essential for maximal transcriptional activation of IFN-responsive genes: it augments promoter recruitment and chromatin binding, supporting antiviral responses, macrophage activation, and inflammation. Aberrant Ser727 phosphorylation is linked to tumorigenesis and impaired IFN signaling by disrupting normal STAT1 activity.

使用情報

Application

WB, FCM

Dilution

WB	FCM
1:10000	1:1000-1:100000

Reactivity

Human

Source

Rabbit Monoclonal Antibody

MW

87 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/18574148/
https://pubmed.ncbi.nlm.nih.gov/12671680/

Application Data

WB

Validated by Selleck

Lane 1: U937 (IL-4 treated), Lane 2: U937