Pinocembrin

製品コードS3941 バッチS394101

印刷

化学情報

Chemical Structure Synonyms Dihydrochrysin, Galangin flavanone, 5,7-Dihydroxyflavanone Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C15H12O4
分子量 256.25 CAS No. 480-39-7
Solubility (25°C)* 体外 DMSO 51 mg/mL (199.02 mM)
Ethanol 51 mg/mL (199.02 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Pinocembrin (Dihydrochrysin, Galangin flavanone, 5,7-Dihydroxyflavanone) is a major flavonoid molecule incorporated as multifunctional in the pharmaceutical industry. Its vast range of pharmacological activities has been well researched including antimicrobial, anti-inflammatory, antioxidant, and anticancer activities.
in vitro Pinocembrin (5,7-dihydroxyflavanone) is one of the primary flavonoids isolated from the variety of plants, mainly from Pinus heartwood, Eucalyptus, Populus, Euphorbia, and Sparattosperma leucanthum, in the diverse flora and purified by various chromatographic techniques. Its vast range of pharmacological activities has been well researched including antimicrobial, anti-inflammatory, antioxidant, and anticancer activities[1]. Pinocembrin has been shown to increase neuronal viability, decrease lactate dehydrogenase release, inhibit the production of NO and ROS, increase glutathione levels, and downregulate the expression of neuronal NO synthase (nNOS) and iNOS in primary cortical neurons subjected to oxygen-glucose deprivation/reoxygenation (OGD/R)[2].
in vivo Pinocembrin can be used as neuroprotective against cerebral ischemic injury with a wide therapeutic time window, which may be attributed to its antiexcitotoxic effects[1]. It has the ability to reduce reactive oxygen species (ROS), protect the BBB, modulate mitochondrial function, and regulate apoptosis. Pinocembrin (10 mg/kg, i.v.) could reduce brain swelling; improve behavioral deficits; and alleviate neuronal apoptosis, edema of astrocytic end-feet, and the deformation of endothelial cells and capillaries. Pinocembrin reduces endoplasmic reticulum (ER) stress and apoptosis by decreasing C/EBP homologous protein (CHOP)/GADD153 and caspase-12 expression via the PERK-elF2α-ATF4 signaling pathway in MCAO rats. LD50 of i.v. pinocembrin in mice is greater than 700 mg/kg[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 SH-SY5Y cells
濃度 4, 8, 20 μM
反応時間 1 h
実験の流れ

SH-SY5Y cells are maintained in DMEM containing 10% FBS, 100 units/mL penicillin, and 100 µg/mL streptomycin in a humidified atmosphere containing 5% CO2 at 37℃.  Cells are passaged by trypsinization every 3-5 days. Then cells are seeded at 1 × 104 cells per well into a 96-well plate and then incubated for 24 h at 37 °C in a humidified atmosphere containing 5% CO2. The entire medium is then replaced with fresh medium containing 1% FBS, and one of the compounds is pretreated for 1 h, followed by the addition of 2.0 µg/mL tunicamycin or 30 nM staurosporine. Nuclear staining assays are carried out after a further 24 h of incubation. Cell death is assessed.
動物実験 動物モデル male Sprague-Dawley rats
投薬量 22.5 or 67.5 mg/kg
投与方法 i.v.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Pinocembrin alleviates chronic morphine-induced analgesic tolerance and hyperalgesia by inhibiting microglial activation [ Neurol Res, 2022, 1-10] PubMed: 35574904
Pinocembrin ameliorates intermittent hypoxia-induced neuroinflammation through BNIP3-dependent mitophagy in a murine model of sleep apnea [ J Neuroinflammation, 2020, 17(1):337] PubMed: 33176803

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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