Datasheet

生物学的記述

Specificity	PKCβ Antibody [B15A17] detects endogenous levels of total PKCβ protein.
Background	Protein kinase C β (PKCβ) is a conventional (classical) serine/threonine kinase isoform within the PKC family that is activated downstream of phospholipase C–coupled receptors and functions as a major node in diacylglycerol (DAG)‑ and Ca²⁺‑dependent signaling cascades controlling cell growth, survival, and differentiation. PKCβ is expressed as two closely related splice variants, PKCβI and PKCβII, which share nearly identical catalytic and regulatory domains but differ at their C‑terminal tails, leading to isoform‑specific localization and substrate preference. Upon activation by DAG, phosphatidylserine, and Ca²⁺, PKCβ translocates to membrane‑associated compartments where it phosphorylates a range of effectors, including MARCKS and other cytoskeletal regulators, and engages pathways linked to glucose transport, cytoskeletal reorganization, and inflammatory signaling. PKCβII is a key component of the B‑cell receptor (BCR) signaling axis, where it modulates B‑cell development, selection, and antibody output, and its overexpression can skew B‑cell subsets and elevate serum IgM. PKCβII is frequently implicated in promoting cell survival, epithelial–mesenchymal transition (EMT), and metastasis, partly through activation of NF‑κB and other pro‑survival transcriptional programs, and pharmacologic inhibition of PKCβ has been explored as a means to suppress tumor growth and chemoresistance in several solid‑tumor models.

Specificity

PKCβ Antibody [B15A17] detects endogenous levels of total PKCβ protein.

Background

Protein kinase C β (PKCβ) is a conventional (classical) serine/threonine kinase isoform within the PKC family that is activated downstream of phospholipase C–coupled receptors and functions as a major node in diacylglycerol (DAG)‑ and Ca²⁺‑dependent signaling cascades controlling cell growth, survival, and differentiation. PKCβ is expressed as two closely related splice variants, PKCβI and PKCβII, which share nearly identical catalytic and regulatory domains but differ at their C‑terminal tails, leading to isoform‑specific localization and substrate preference. Upon activation by DAG, phosphatidylserine, and Ca²⁺, PKCβ translocates to membrane‑associated compartments where it phosphorylates a range of effectors, including MARCKS and other cytoskeletal regulators, and engages pathways linked to glucose transport, cytoskeletal reorganization, and inflammatory signaling. PKCβII is a key component of the B‑cell receptor (BCR) signaling axis, where it modulates B‑cell development, selection, and antibody output, and its overexpression can skew B‑cell subsets and elevate serum IgM. PKCβII is frequently implicated in promoting cell survival, epithelial–mesenchymal transition (EMT), and metastasis, partly through activation of NF‑κB and other pro‑survival transcriptional programs, and pharmacologic inhibition of PKCβ has been explored as a means to suppress tumor growth and chemoresistance in several solid‑tumor models.

使用情報

Application

WB, IP, FCM

Dilution

WB	IP	FCM
1:1000	1:50	1:200

Reactivity

Human, Mouse, Rat

Source

Rabbit Monoclonal Antibody

MW

80 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/20439192/
https://academic.oup.com/jb/article-abstract/132/5/677/919796

Application Data

WB

Validated by Selleck

Lane 1: K562, Lane 2: Raji