PMA chemical (Phorbol 12-myristate 13-acetate)

製品コードS7791 バッチS779103

化学情報

	Synonyms	12-O-Tetradecanoylphorbol-13-acetate,TPA, Phorbol myristate acetate	Storage (From the date of receipt)	3 years -20°C(in the dark) powder
	化学式	C₃₆H₅₆O₈
	分子量	616.83	CAS No.	16561-29-8
Solubility (25°C)*	体外	DMSO	100 mg/mL (162.11 mM)
		Ethanol	100 mg/mL (162.11 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	PKCの強力な活性化剤であるPMA (Phorbol 12-myristate 13-acetate)は、ナノモル濃度で活性を示します。Phorbol 12-myristate 13-acetate (PMA)は、sphingosine-1-phosphate (S1P)を誘導します。
in vitro	In endothelial cells, PMA (Phorbol 12-myristate 13-acetate) increases levels of Thy-1 mRNA and protein, induces Thy-1 up-regulation and inhibits capillary-like tube formation and endothelial cell migration.
in vivo	In the zebrafish model, PMA (Phorbol 12-myristate 13-acetate) initially enhances endothelial cell migration, subsequently activating the PKC-δ/Syk/NF-κB-mediated pathway to up-regulate Thy-1, which in turn inhibits endothelial cell migration.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Human umbilical vein endothelial cells (HUVECs), human dermal microvascular endothelial cells (HDMECs), human pulmonary microvascular endothelial cell line (HPMECs)
	濃度	20 ng/mL
	反応時間	14, 16 and 18 h for levels of Ty-1 mRNA, 20, 24 and 28 h for levels of Ty-1 protein
	実験の流れ	After transfection with Ty-1 promoter plasmid (0.5 μg) and Renilla plasmid (50ng), the cells are treated with PMA (Phorbol 12-myristate 13-acetate) at 20 ng/mL, washed with ice-cold phosphate-buffered saline (PBS), and then lysed in passive lysis buffer. Luciferase activity is measured using the Dual-Luciferase Reporter Assay kit.
動物実験	動物モデル	Transgenic Tg(fi1:EGFP)y1 embryos of zebrafsh (Danio rerio)
	投薬量	20 ng/mL
	投与方法	--

参考

https://pubmed.ncbi.nlm.nih.gov/30389973/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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