PMSF

製品コードS3025 バッチS302503

印刷

化学情報

Chemical Structure Synonyms Phenylmethylsulfonyl Fluoride, Benzylsulfonyl fluoride Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C7H7FO2S
分子量 174.19 CAS No. 329-98-6
Solubility (25°C)* 体外 DMSO 38 mg/mL (218.15 mM)
Ethanol 38 mg/mL (218.15 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 PMSF (Phenylmethylsulfonyl Fluoride, Benzylsulfonyl fluoride) is an irreversible serine/cysteine protease inhibitor.
in vitro Although human trypsin is less susceptible to inhibition by PMSF, this compound rapidly inactivates purified chymotrypsin from human pancreas. It also rapidly inhibits acetylcholinesterase from human red cells. [1] As an inhibitor of phosphatidylinositol-specific phospholipase C, treatment with this chemical at 2 mM almost completely inhibits carbachol-stimulated inositol incorporation into phosphatidylinositol (PI) of longitudinal smooth muscle of guinea pig ileum, while it has no effect on potassium-stimulated inositol incorporation. In contrast to its specific inhibition of carbachol-stimulated phosphoinositide turnover, this agent produces a transient inhibition of contraction by both carbachol and potassium. [3] It has been shown to inhibit the addition of ethanolamine phosphate to glycosylphosphatidylinositol (GPI) intermediates in Trypanosoma brucei. This inhibitor also inhibits the acylation of the inositol residue of GPI intermediates in bloodstream form T. brucei. It inhibits ethanolamine phosphate addition and inositol acylation for procyclic forms of T. brucei but not for mammalian HeLa cells. [4] This compound is the more reactive inactivator of mouse acetylcholinesterase (AChE), as the 8-fold higher BSF concentration is necessary to achieve even a 6-fold slower inactivation than that using PMSF. [7]
in vivo Intraperitoneal injection of PMSF produces dose-dependent analgesia in Sprague-Dawley rats. This compound significantly enhances the analgesic effect of beta-endorphin (END) in rats. [2] Mice receiving i.p. injections of this chemical exhibit cannabinoid effects that include antinociception, hypothermia and immobility with ED50 of 86 mg/kg, 224 mg/kg and 206 mg/kg, respectively. Pretreatment with an inactive dose of this compound (30 mg/kg) enhances the effects of anandamide on tail-flick response (antinociception), spontaneous activity and mobility by 5-, 10- and 8-fold, respectively. [5] Administration of this chemical 12 hours prior to PSP causes complete protection in organophosphorus ester-induced delayed neuropathy (OPIDN) in hens, but it administered 4 hours after PSP potentiates its neurotoxic effects. [6] Pretreatment with this compound (30 mg/kg, i.p.) prior to an injection of 1 or 10 mg/kg 3H-anandamide results 5 minutes later in enhanced brain levels of anandamide compared to those obtained with 3H-anandamide plus vehicle injection. [8] Pretreatment with it inhibits tri-ortho-cresyl phosphate (TOCP)-induced neurofilament (NF) degradation, and protects hens against the development of organophosphate-induced delayed neuropathy (OPIDN). [9] Administration of this chemical enhances the characteristic cannabimimetic effects of Δ(9)-tetrahydrocannabinol (THC) or anandamide (AEA) in ICR mice, by inhibiting the enzyme fatty acid amide hydrolase. [10]

プロトコル(参考用のみ)

動物実験 動物モデル Male ICR mice subjected to anandamide injection
投薬量 ~1000 mg/kg
投与方法 Administered i.p. 10 minutes before i.v. anandamide injection

参考

  • https://pubmed.ncbi.nlm.nih.gov/4306345/
  • https://pubmed.ncbi.nlm.nih.gov/6292607/
  • https://pubmed.ncbi.nlm.nih.gov/6090026/
  • https://pubmed.ncbi.nlm.nih.gov/7518442/
  • https://pubmed.ncbi.nlm.nih.gov/9399986/
  • https://pubmed.ncbi.nlm.nih.gov/10591511/

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Intracellular complement C5a/C5aR1 stabilizes β-catenin to promote colorectal tumorigenesis [ Cell Rep, 2022, 39(9):110851] PubMed: 35649359
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A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma [ Mol Med Rep, 2022, 26(2)255] PubMed: 35703357

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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