受注:045-509-1970 |
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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C19H16N2O3 |
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分子量 | 320.34 | CAS No. | 113906-27-7 | |
Solubility (25°C)* | 体外 | DMSO | 64 mg/mL (199.78 mM) | |
Ethanol | 5 mg/mL (15.6 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | PNU-75654 disrupts the Wnt signaling pathway via inhibition of the interaction (KD50=450 nM) between β-catenin and Tcf4. |
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in vitro | The PNU-74654 (PNU) compound is a non-FDAapproved drug which prevents that Tcf from binding to beta-catenin, acting as a Wnt/beta-catenin antagonist. In NCI-H295 cells,PNU-74654 significantly decreases cell proliferation 96 h after treatment, increases early and late apoptosis, decreases nuclear betacatenin accumulation, impairs CTNNB1/beta-catenin expression and increases betacatenin target genes 48 h after treatment. No effects are observed on HeLa cells. In NCI-H295 cells, PNU-74654 decreases cortisol, testosterone and androstenedione secretion 24 and 48 h after treatment. Additionally, in NCI-H295 cells, PNU-74654 decreases SF1 and CYP21A2 mRNA expression as well as the protein levels of STAR and aldosterone synthase 48 h after treatment. In Y1 cells, PNU-74654 impairs corticosterone secretion 24 h after treatment but does not decrease cell viability[2]. |
細胞アッセイ | 細胞株 | Adrenal (NCI-H295 and Y1) and non-adrenal (HeLa) cell lines |
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濃度 | 5-200 μM | |
反応時間 | 24-96 h | |
実験の流れ | NCI-H295 cells are plated at 200,000 cells per well in 24-well plates for gene expression, protein analysis and adrenal steroid measurements. After 48 h, cells are treated with vehicle (0.1%-0.4% DMSO) or 10, 50, 100 and 200 μM PNU-74654. After 24 and 48 h, medium supernatants are collected for adrenal steroid measurements. Adherent cells are fixed for immunofluorescence or harvested for RNA and protein isolation. |
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Protective effects of emodin on subchondral bone and articular cartilage in osteoporotic osteoarthritis rats: A preclinical study [ Exp Gerontol, 2024, 190:112413] | PubMed: 38570055 |
GRIK5 stimulates colon cancer growth and metastasis through cAMP/PKA/CADM3 signaling [ Cell Biol Int, 2023, 47(7):1259-1266] | PubMed: 36959746 |
A specific upregulated lncRNA in colorectal cancer promotes cancer progression [ JCI Insight, 2022, e158855] | PubMed: 35617032 |
β-catenin regulates HIV latency and modulates HIV reactivation [ PLoS Pathog, 2022, 18(3):e1010354] | PubMed: 35255110 |
Tankyrase Regulates Neurite Outgrowth through Poly(ADP-ribosyl)ation-Dependent Activation of β-Catenin Signaling [ Int J Mol Sci, 2022, 23(5)2834] | PubMed: 35269974 |
C-Peptide Promotes Cell Migration by Controlling Matrix Metallopeptidase-9 Activity Through Direct Regulation of β-Catenin in Human Endometrial Stromal Cells [ Front Cell Dev Biol, 2022, 10:800181] | PubMed: 35127683 |
MDMX acts as a pervasive preleukemic-to-acute myeloid leukemia transition mechanism [ Cancer Cell, 2021, 39(4):529-547.e7] | PubMed: 33667384 |
β-catenin-promoted cholesterol metabolism protects against cellular senescence in naked mole-rat cells [ Commun Biol, 2021, 4(1):357] | PubMed: 33742113 |
Inhibition of Aurora kinase A activity enhances the antitumor response of beta-catenin blockade in human adrenocortical cancer cells [ Mol Cell Endocrinol, 2021, 528:111243] | PubMed: 33716050 |
A PROTAC peptide induces durable β-catenin degradation and suppresses Wnt-dependent intestinal cancer [ Cell Discov, 2020, 6:35] | PubMed: 32550000 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。