Datasheet

生物学的記述

Specificity	Profilin-1 Antibody [P24C16] detects endogenous levels of total Profilin-1 protein.
Background	Profilin-1 serves as the ubiquitously expressed member of the profilin family of actin-monomer binding proteins, acting as a central regulator of actin cytoskeleton dynamics essential for cellular motility, division, and adhesion. It features a compact globular fold with distinct actin-binding, polyproline ligand (PLP), and phosphoinositide-binding sites that enable G-actin sequestration and nucleotide exchange from ADP to ATP states. Profilin-1 delivers ATP-G-actin to the barbed ends of growing filaments while discriminating against spontaneous nucleation, preferentially channeling monomers to Ena/VASP and formin pathways over Arp2/3 complex assembly; this creates homeostatic balance by inhibiting Arp2/3-driven lamellipodia while promoting VASP-mediated leading-edge protrusion through adhesion-dependent PKA-regulated interactions that enhance F-actin accumulation without altering total cellular levels. Profilin-1 also binds polyproline motifs in palladin and dynamin-1 to coordinate actin-membrane remodeling and endocytosis, with phosphorylation at S137 fine-tuning affinity for PLP partners like VASP to sustain directional migration. Profilin-1 governs embryonic cleavage and blastocyst formation by ensuring actin flux for cytokinesis and polarity, while in migrating cells, it dictates protrusion-retraction cycles critical for immune surveillance and wound healing, making it ideal for researchers probing actin pathway competition where isoform redundancy reveals context-specific roles. Dysregulation through depletion elevates Arp2/3 activity, disrupts homeostasis, and triggers embryonic lethality by the 2-cell stage, while mutations impair neuronal trafficking in ALS contexts.

Specificity

Profilin-1 Antibody [P24C16] detects endogenous levels of total Profilin-1 protein.

Background

Profilin-1 serves as the ubiquitously expressed member of the profilin family of actin-monomer binding proteins, acting as a central regulator of actin cytoskeleton dynamics essential for cellular motility, division, and adhesion. It features a compact globular fold with distinct actin-binding, polyproline ligand (PLP), and phosphoinositide-binding sites that enable G-actin sequestration and nucleotide exchange from ADP to ATP states. Profilin-1 delivers ATP-G-actin to the barbed ends of growing filaments while discriminating against spontaneous nucleation, preferentially channeling monomers to Ena/VASP and formin pathways over Arp2/3 complex assembly; this creates homeostatic balance by inhibiting Arp2/3-driven lamellipodia while promoting VASP-mediated leading-edge protrusion through adhesion-dependent PKA-regulated interactions that enhance F-actin accumulation without altering total cellular levels. Profilin-1 also binds polyproline motifs in palladin and dynamin-1 to coordinate actin-membrane remodeling and endocytosis, with phosphorylation at S137 fine-tuning affinity for PLP partners like VASP to sustain directional migration. Profilin-1 governs embryonic cleavage and blastocyst formation by ensuring actin flux for cytokinesis and polarity, while in migrating cells, it dictates protrusion-retraction cycles critical for immune surveillance and wound healing, making it ideal for researchers probing actin pathway competition where isoform redundancy reveals context-specific roles. Dysregulation through depletion elevates Arp2/3 activity, disrupts homeostasis, and triggers embryonic lethality by the 2-cell stage, while mutations impair neuronal trafficking in ALS contexts.

使用情報

Application

WB

Dilution

WB

1:1000

Reactivity

Human, Mouse, Rat, Hamster, Monkey, Dog

Source

Rabbit Monoclonal Antibody

MW

15 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/25543281/
https://pubmed.ncbi.nlm.nih.gov/24385538/

Application Data

WB

Validated by Selleck

Lane 1: Hela, Lane 2: COS, Lane 3: C6, Lane 4: CHO