受注:045-509-1970 |
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Synonyms | Dinoprostone | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C20H32O5 |
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分子量 | 352.47 | CAS No. | 363-24-6 | ||||
Solubility (25°C)* | 体外 | DMSO | 70 mg/mL (198.59 mM) | ||||
Ethanol | 70 mg/mL (198.59 mM) | ||||||
Water | 2.5 mg/mL (7.09 mM) | ||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Prostaglandin E2 (PGE2, Dinoprostone) plays important effects in labour (softens cervix and causes uterine contraction) and also stimulates osteoblasts to release factors that stimulate bone resorption by osteoclasts. |
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in vitro | PGE2 acts via EP4 receptors on osteoclasts during the progression of OA and OA-related pain.[1] |
in vivo | PGE2 (0.3 μg/k, i.p.) significantly reduces the number of peritoneab macrophages undergoing phagocytosis of the methacrybate microbeads in rats. |
細胞アッセイ | 細胞株 | Mice osteoclasts differentiated from bone marrow macrophages (BMMs) |
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濃度 | 1, 10, 100, 1000, 10000 nM | |
反応時間 | 18 h | |
実験の流れ | When BMMs differentiated into mature osteoclasts, a TRAP staining kit is used to stain mature osteoclasts according to the manufacturer s instructions, and TRAP-positive osteoclasts with 5 or more nuclei are counted. For BMM migration, BMMs (1×104 cells per well) are seeded in the upper chamber of the Transwell inserts with or without inhibitors and antagonists. PGE2 is added in lower chamber. The culture medium is the same between the upper chamber and lower chamber in the Transwell. After 18 h, the cells are fixed with 4% PFA and stained with crystal violet. The cells on the bottom of the Transwell insert are used to assess migration. At least five fields of view per insert are photographed, and migrated cells are counted. |
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動物実験 | 動物モデル | Male Sprague Dawley rats |
投薬量 | 0.3 µg/kg | |
投与方法 | i.p. |
Anti-angiogenic therapy using the multi-tyrosine kinase inhibitor Regorafenib enhances tumor progression in a transgenic mouse model of ß-cell carcinogenesis [ Br J Cancer, 2023, 10.1038/s41416-023-02389-6] | PubMed: 37620408 |
Anti-angiogenic therapy using the multi-tyrosine kinase inhibitor Regorafenib enhances tumor progression in a transgenic mouse model of ß-cell carcinogenesis [ Br J Cancer, 2023, 129(8):1225-1237] | PubMed: 37620408 |
Stabilization of E-cadherin adhesions by COX-2/GSK3β signaling is a targetable pathway in metastatic breast cancer [ JCI Insight, 2023, 8(6)e156057] | PubMed: 36757813 |
The cyclooxygenase-expressing mesenchyme resists intestinal epithelial injury by paracrine signaling [ Cell Regen, 2023, 12(1):30] | PubMed: 37574502 |
The cyclooxygenase-expressing mesenchyme resists intestinal epithelial injury by paracrine signaling [ Cell Regen, 2023, 12(1):30] | PubMed: 37574502 |
PGE2 activates EP4 in subchondral bone osteoclasts to regulate osteoarthritis [ Bone Res, 2022, 10(1):27] | PubMed: 35260562 |
Macrophages evoke autophagy of hepatic stellate cells to promote liver fibrosis in NAFLD mice via the PGE2/EP4 pathway [ Cell Mol Life Sci, 2022, 79(6):303] | PubMed: 35588334 |
CD96 Downregulation Promotes the Immune Response of CD4 T Cells and Associates with Ankylosing Spondylitis [ Biomed Res Int, 2022, 2022:3946754] | PubMed: 35769669 |
Cross-species single-cell transcriptomic analysis reveals divergence of cell composition and functions in mammalian ileum epithelium [ Cell Regen, 2022, 11(1):19] | PubMed: 35511361 |
Inhibition of cyclooxygenase-2 enhanced intestinal epithelial homeostasis via suppressing β-catenin signalling pathway in experimental liver fibrosis [ J Cell Mol Med, 2021, 10.1111/jcmm.16730] | PubMed: 34145945 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。