Prostaglandin E2 (PGE2)

製品コードS3003 バッチS300303

印刷

化学情報

Chemical Structure Synonyms Dinoprostone Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C20H32O5
分子量 352.47 CAS No. 363-24-6
Solubility (25°C)* 体外 DMSO 70 mg/mL (198.59 mM)
Ethanol 70 mg/mL (198.59 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Prostaglandin E2 (PGE2, Dinoprostone) plays important effects in labour (softens cervix and causes uterine contraction) and also stimulates osteoblasts to release factors that stimulate bone resorption by osteoclasts. It is easy to discolor when stored in liquid environment for a long time. It is recommended to store it in powder form and try to use it as soon as possible!
in vitro

PGE2 acts via EP4 receptors on osteoclasts during the progression of OA and OA-related pain.[1]
in vivo

PGE2 (0.3 μg/k, i.p.) significantly reduces the number of peritoneab macrophages undergoing phagocytosis of the methacrybate microbeads in rats.

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Mice osteoclasts differentiated from bone marrow macrophages (BMMs)
濃度 1, 10, 100, 1000, 10000 nM
反応時間 18 h
実験の流れ

When BMMs differentiated into mature osteoclasts, a TRAP staining kit is used to stain mature osteoclasts according to the manufacturer s instructions, and TRAP-positive osteoclasts with 5 or more nuclei are counted. For BMM migration, BMMs (1×104 cells per well) are seeded in the upper chamber of the Transwell inserts with or without inhibitors and antagonists. PGE2 is added in lower chamber. The culture medium is the same between the upper chamber and lower chamber in the Transwell. After 18 h, the cells are fixed with 4% PFA and stained with crystal violet. The cells on the bottom of the Transwell insert are used to assess migration. At least five fields of view per insert are photographed, and migrated cells are counted.
動物実験 動物モデル Male Sprague Dawley rats
投薬量 0.3 µg/kg
投与方法 i.p.

参考

  • https://pubmed.ncbi.nlm.nih.gov/6804558/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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