Protocatechuic acid

製品コードS3975 バッチS397501

化学情報

	Synonyms	3,4-Dihydroxybenzoic acid, Protocatechuate	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₇H₆O₄
	分子量	154.12	CAS No.	99-50-3
Solubility (25°C)*	体外	DMSO	30 mg/mL (194.65 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Protocatechuic acid (PCA, 3,4-Dihydroxybenzoic acid, Protocatechuate), a dihydroxybenzoic acid, is a type of widely distributed naturally occurring phenolic acid.
in vitro	Protocatechuic acid (PCA) inhibits cell migration and invasion at non-cytotoxic concentrations. It down-regulates the Ras/Akt/NF-κB pathway by targeting RhoB activation, which in turn leads to a reduction of MMP-mediated cellular events in cancer cells. In some cancer cells, PCA treatment suppresses cell adhesion and the production of interleukin (IL)-6, IL-8, vascular endothelial growth factor and the intercellular adhesion molecule-1, which would further attenuate angiogenic and metastatic actions^[1]. PCA will lower the expression of cleaved caspase-3 and decrease the rate of cardiomyocyte apoptosis induced by hypoxia/reoxygenation. PCA is proven to have the neuroprotective effects in PC12 cells^[2].
in vivo	Protocatechuic acid (PCA) inhibits metastasis of B16/F10 melanoma cells to the liver in mice^[1]. PCA is seen to enhance learning and memory ability, and alleviate oxidative stress, apoptosis and glial proliferation following CIH exposure in rats. In addition, PCA administration also decreases the level of IL-1β in brain and increases the expression of BDNF and SYN. Its administration inhibits apoptosis by decreasing JNK/P38 phosphoryltion and the downstream target including c-fos, Bax and Cleaved Caspase-3 in the hippocampal and prefrontal cortex areas. PCA exerts significant neuroprotective effects in CIH rats^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	human gastric adenocarcinoma (AGS) cells
	濃度	1 mM
	反応時間	24, 48 and 72 h
	実験の流れ	Cells (1 × 10⁴ cells/mL) are seeded in 12 well plates and treated with PCA (1.0 mM) or dimethyl sulphoxide (DMSO) as control for 24, 48 and 72 h. The number of cells is measured by the Trypan blue dye exclusion assay.
動物実験	動物モデル	6-week-old C57/BL6 mice with B16/F10 melanoma
	投薬量	200 and 400 mg/kg
	投与方法	oral administration

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Cinnamic acid inhibits cell viability, invasion, and glycolysis in primary endometrial stromal cells by suppressing NF-κB-induced transcription of PKM2 [ Biosci Rep, 2021, BSR20211828]	PubMed: 34499120

Cinnamic acid inhibits cell viability, invasion, and glycolysis in primary endometrial stromal cells by suppressing NF-κB-induced transcription of PKM2 [ Biosci Rep, 2021, BSR20211828]

PubMed: 34499120

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。