Psoralen

製品コードS4737 バッチS473701

化学情報

	Synonyms	Psoralene, Ficusin, Furocoumarin	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₁H₆O₃
	分子量	186.16	CAS No.	66-97-7
Solubility (25°C)*	体外	DMSO	37 mg/mL (198.75 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Psoralen (Psoralene, Ficusin, Furocoumarin) is a naturally occurring furocoumarin that intercalates with DNA, inhibiting DNA synthesis and cell division.
in vitro	Psoralen inhibits the proliferation of MCF-7/ADR cells as shown by G0/G1 phase arrest rather than encouraging apoptosis. Psoralen reverses MDR(multidrug resistance) through inhibiting ATPase activity rather than reducing P-gp expression. Psoralen inhibits the migration abilities of MCF-7/ADR cells by repressing EMT possibly through inhibiting the activation of NF-κB. Psoralens are photoactive compounds that readily alkylate DNA when activated by longwave ultraviolet light. Proliferation has been significantly promoted in MCF-7/ADR cells treated with low concentration of psoralen (<10.75 µM) and inhibited with high concentration(>21.5 µM). Psoralen can inhibit metastasis of breast cancer. Psoralen mediates a variety of cell processes including cell death, proliferation, inflammation and migration^[1].
in vivo	Psoralen has been characterized as a tumor suppressor in various tumors^[1]. Psoralen ameliorates sex hormone deficiency-induced osteoporosis in female and male mice. It has antiosteoporosis effect in ovariectomy-induced osteoporotic rats via stimulating osteoblastic differentiation from bone mesenchymal stem cells^[2].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Human breast cancer MCF-7/ADR cells, MCF-10A, and MCF-7 cells
	濃度	0, 21.5, 43.0, 64.5, 86.0, 107.5 µM
	反応時間	48 h
	実験の流れ	The effects of psoralen on cell proliferation are measured by MTT assay. MCF-10A and MCF-7/ADR cells are cultured in 96-well plates at a cell density of 2×10⁴ cells per well for 48 h. The medium is then removed and replaced by fresh medium containing different concentrations of psoralen (0, 21.5, 43.0, 64.5, 86.0, 107.5 μM) for 48 h. Cells in the negative control group are incubated with RPMI-1640 culture medium supplemented with 0.1% dimethyl sulfoxide (DMSO). Cells are incubated with 10 µL MTT (5 mg/mL) for 4 h, and then discarded the medium and added 200 µL DMSO. The spectrophotometric absorbance is measured at 490 nm with enzyme-labeling instrument after the crystals were fully dissolved.
動物実験	動物モデル	ICR mice
	投薬量	10 mg/kg and 20 mg/kg
	投与方法	intragastrically

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Coumarins of Lovage Roots (Levisticum officinale W.D.J.Koch): LC-MS Profile, Quantification, and Stability during Postharvest Storage [ Metabolites, 2022, 13(1)3]	PubMed: 36676932

Coumarins of Lovage Roots (Levisticum officinale W.D.J.Koch): LC-MS Profile, Quantification, and Stability during Postharvest Storage [ Metabolites, 2022, 13(1)3]

PubMed: 36676932

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。