Puromycin

製品コードE4996 バッチE499601

印刷

化学情報

Chemical Structure Synonyms CL13900, Puromycine Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C22H29N7O5
分子量 471.51 CAS No. 53-79-2
Solubility (25°C)* 体外 DMSO 94 mg/mL (199.35 mM)
Ethanol 4 mg/mL (8.48 mM)
Water Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 4.700mg/ml (9.97mM) Taking the 1 mL working solution as an example, add 50 μL of 94 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil

この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。

 0.450mg/ml (0.95mM) Taking the 1 mL working solution as an example, add 50 μL of 9 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Puromycin (CL13900, Puromycine) is an antibiotic, analog of the 3' end of aminoacyl-tRNA and an inhibitor of protein synthesis that mimics tyrosyl-tRNA, causing premature termination of translation. It blocks translation by labeling and releasing elongating polypeptide chains from ribosomes and has been widely used in molecular biology research.

プロトコル(参考用のみ)

参考

  • https://pubmed.ncbi.nlm.nih.gov/12732187/#&dopt=Abstract
  • https://pubmed.ncbi.nlm.nih.gov/10666460/#&dopt=Abstract

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Alterations in PD-L1 succinylation shape anti-tumor immune responses in melanoma [ Nat Genet, 2025, 57(3):680-693] PubMed: 40069506
CD137L promotes immune surveillance in melanoma via HLTF regulation [ Nat Commun, 2025, 16(1):8478] PubMed: 41006211
BRD8 Guards the Pluripotent State by Sensing and Maintaining Histone Acetylation [ Adv Sci (Weinh), 2025, 12(5):e2409160] PubMed: 39656858
Ints7 deficiency activates DNA damage response to elicit resurgence of endogenous retrovirus MERVL and anastasis of embryonic stem cells [ Nucleic Acids Res, 2025, 53(15)gkaf797] PubMed: 40842237
ELTD1 inhibits differentiation of hemogenic endothelium progenitors from human embryonic stem cells through the HPIP-Wnt pathway [ Exp Mol Med, 2025, 57(6):1216-1231] PubMed: 40456828
A novel pathway for stemness propagation and chemoresistance in non-small cell lung cancer via phosphorylated PKM2-loaded small extracellular vesicles [ Theranostics, 2025, 15(8):3439-3461] PubMed: 40093893
The de novo DNA methyltransferase 3B is a novel epigenetic regulator of MYC in multiple myeloma, representing a promising therapeutic target to counter relapse [ J Exp Clin Cancer Res, 2025, 44(1):125] PubMed: 40241199
Keratin 6A promotes skin inflammation through JAK1-STAT3 activation in keratinocytes [ J Biomed Sci, 2025, 32(1):47] PubMed: 40346694
Unveiling the mechanisms of CEBPD-orchestrated TAM polarization through RGS2/PAR2 pathway and its impact on ICB efficacy in clear cell renal cell carcinoma [ J Immunother Cancer, 2025, 13(7)e010898] PubMed: 40659446
Repurposing MDM2 inhibitor RG7388 for TP53-mutant NSCLC: a p53-independent pyroptotic mechanism via ROS/p-p38/NOXA/caspase-3/GSDME axis [ Cell Death Dis, 2025, 16(1):452] PubMed: 40523886

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。