Puromycin aminonucleoside

製品コードS9631 バッチS963101

印刷

化学情報

Synonyms

NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₂H₁₈N₆O₃

分子量

294.31

CAS No.

58-60-6

Solubility (25°C)*

体外

DMSO

59 mg/mL (200.46 mM)

Water

30 mg/mL (101.93 mM)

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2.950mg/ml (10.02mM)	Taking the 1 mL working solution as an example, add 50 μL of 59 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.490mg/ml (1.66mM)	Taking the 1 mL working solution as an example, add 50 μL of 9.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Puromycin aminonucleoside (NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN), the aminonucleoside portion of the antibiotic puromycin, is a reversible inhibitor of dipeptidyl-peptidase II and cytosol alanyl aminopeptidase that induces apoptosis in mesangial cells (MCs) accompanied by declined cell viability and enhanced inflammatory response.Puromycin aminonucleoside can be used to induce animal models of Kidney Disease.
in vitro	Puromycin aminonucleoside (PAN) induces MC apoptosis accompanied by the declined cell viability and enhanced inflammatory response. In this compound-treated MCs, ERRα overexpression further aggravates PAN-induced apoptosis.^[1]
in vivo	In agreement with the in vitro study, increased ERRα expression is observed in line with enhanced apoptotic response in renal cortex from PAN-treated rats.^[1]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	mouse mesangial cells
	濃度	50 μg/ml, 100 μg/ml
	反応時間	24 h
	実験の流れ	For apoptosis assay, mesangial cells are treated with this compound for 24 h at the indicated concentration. After treatment, cells are stained with FITC-Annexin V and PI according to the manufacturer’s protocol. Cell apoptosis is determined using a BD FACS Calibur flow cytometer and data is analyzed with Flowjo software.
動物実験	動物モデル	male Sprague-Dawley rats
	投薬量	150 mg/kg
	投与方法	IV

参考

https://pubmed.ncbi.nlm.nih.gov/30698047/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The tegument protein VP22 of pseudorabies virus inhibits cGAS condensation by inducing nuclear-to-cytoplasmic translocation of DDX21 [ PLoS Pathog, 2025, 21(9):e1013549]	PubMed: 41021645
DFFB suppresses interferon to enable cancer persister cell regrowth [ bioRxiv, 2025, 2025.08.15.670603]	PubMed: 40894800
Suppression of ZBP1-mediated NLRP3 inflammasome by the tegument protein VP22 facilitates pseudorabies virus infection [ mBio, 2024, 15(12):e0194524]	PubMed: 39475237
Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO [ Cell Mol Biol Lett, 2023, 28(1):62]	PubMed: 37525118
Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO [ Cell Mol Biol Lett, 2023, 28(1):62]	PubMed: 37525118
SNAI2 promotes the malignant transformation of oral leukoplakia by modulating p-EMT [ Oral Dis, 2022, 10.1111/odi.14321]	PubMed: 35894087
PD-L1 confers glioblastoma multiforme malignancy via Ras binding and Ras/Erk/EMT activation [ Biochim Biophys Acta Mol Basis Dis, 2018, 1864(5 Pt A):1754-1769]	PubMed: 29510196

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。