受注:045-509-1970 |
技術サポート:[email protected] 平日9:00〜18:00 1営業日以内にご連絡を差し上げます |
Synonyms | NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C12H18N6O3 |
|||
分子量 | 294.31 | CAS No. | 58-60-6 | |
Solubility (25°C)* | 体外 | DMSO | 59 mg/mL (200.46 mM) | |
Water | 30 mg/mL (101.93 mM) | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Puromycin aminonucleoside (NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN), the aminonucleoside portion of the antibiotic puromycin, is a reversible inhibitor of dipeptidyl-peptidase II and cytosol alanyl aminopeptidase that induces apoptosis in mesangial cells (MCs) accompanied by declined cell viability and enhanced inflammatory response. |
---|---|
in vitro | Puromycin aminonucleoside (PAN) induces MC apoptosis accompanied by the declined cell viability and enhanced inflammatory response. In PAN-treated MCs, ERRα overexpression further aggravates PAN-induced apoptosis.[1] |
in vivo | In agreement with the in vitro study, increased ERRα expression is observed in line with enhanced apoptotic response in renal cortex from PAN-treated rats.[1] |
細胞アッセイ | 細胞株 | mouse mesangial cells |
---|---|---|
濃度 | 50 μg/ml, 100 μg/ml | |
反応時間 | 24 h | |
実験の流れ | For apoptosis assay, mesangial cells are treated with PAN for 24 h at the indicated concentration. After treatment, cells are stained with FITC-Annexin V and PI according to the manufacturer’s protocol. Cell apoptosis is determined using a BD FACS Calibur flow cytometer and data is analyzed with Flowjo software. |
|
動物実験 | 動物モデル | male Sprague-Dawley rats |
投薬量 | 150 mg/kg | |
投与方法 | IV |
Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO [ Cell Mol Biol Lett, 2023, 28(1):62] | PubMed: 37525118 |
Peptidase inhibitor (PI16) impairs bladder cancer metastasis by inhibiting NF-κB activation via disrupting multiple-site ubiquitination of NEMO [ Cell Mol Biol Lett, 2023, 28(1):62] | PubMed: 37525118 |
SNAI2 promotes the malignant transformation of oral leukoplakia by modulating p-EMT [ Oral Dis, 2022, 10.1111/odi.14321] | PubMed: 35894087 |
PD-L1 confers glioblastoma multiforme malignancy via Ras binding and Ras/Erk/EMT activation [ Biochim Biophys Acta Mol Basis Dis, 2018, 1864(5 Pt A):1754-1769] | PubMed: 29510196 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。