Pyridostatin Trifluoroacetate Salt

製品コードS7444 バッチS744401

印刷

化学情報

Synonyms

RR82 Trifluoroacetate Salt

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₃₁H₃₂N₈O₅·C₂HF₃O₂

分子量

710.7

CAS No.

1472611-44-1

Solubility (25°C)*

体外

DMSO

100 mg/mL (140.7 mM)

Water

100 mg/mL (140.7 mM)

Ethanol

30 mg/mL (42.21 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Pyridostatin Trifluoroacetate Salt is a G-quadruplexe stabilizer with K_d of 490 nM in a cell-free assay, which targets a series of proto-oncogenes.
in vitro	Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC. ^[2] Pyridostatin decreases EBNA1 synthesis via inhibition of G-quadruplexes. ^[3]
in vivo

製品説明

Pyridostatin Trifluoroacetate Salt is a G-quadruplexe stabilizer with K_d of 490 nM in a cell-free assay, which targets a series of proto-oncogenes.

in vitro

Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC. ^[2]

Pyridostatin decreases EBNA1 synthesis via inhibition of G-quadruplexes. ^[3]

in vivo

プロトコル(参考用のみ)

キナーゼアッセイ
キナーゼアッセイ
細胞アッセイ	細胞株	MRC-5–SV40 cells and various cancer cell lines
	濃度	10 μM
	反応時間	48 h
	実験の流れ	Cells are plated at equal confluence and are left either untreated or were treated with 2 μM pyridostatin continually during the indicated time before harvesting the cells. Cells from individual plates are trypsinized and counted in a Coulter counter. Graphs represent the total cell numbers at each time interval, and the error bars represent the s.e.m. Data represent three independent experiments.
動物実験	動物モデル	CB17‐SCID mice
	投薬量	7.5 mg/kg
	投与方法	i.v.

参考

https://pubmed.ncbi.nlm.nih.gov/21941250/
https://pubmed.ncbi.nlm.nih.gov/22306580/
https://pubmed.ncbi.nlm.nih.gov/24633353/

https://pubmed.ncbi.nlm.nih.gov/35107878/

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Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Naphthalene diimide-naphthalimide dyads promote telomere damage by selectively targeting multimeric G-quadruplexes [ Nucleic Acids Res, 2025, 53(7)gkaf301]	PubMed: 40239997
Pyridine-bis(benzimidazole) induces DNA damage at G-quadruplex loci and promotes synthetic lethality with DNA repair inhibition [ Nucleic Acids Res, 2025, 53(12)gkaf543]	PubMed: 40568940
Genomic context influences translesion synthesis DNA polymerase-dependent mechanisms of micronuclei induction by G-quadruplexes [ Cell Rep, 2025, 44(5):115706]	PubMed: 40349342
Polyubiquitinated PCNA triggers SLX4-mediated break-induced replication in alternative lengthening of telomeres (ALT) cancer cells [ Nucleic Acids Res, 2024, gkae785]	PubMed: 39291733
BRCA1 and 53BP1 regulate reprogramming efficiency by mediating DNA repair pathway choice at replication-associated double-strand breaks [ Cell Rep, 2024, 43(4):114006]	PubMed: 38554279
Investigating G-quadruplex structures in RPGR gene: Implications for understanding X-linked retinal degeneration [ Heliyon, 2024, 10(8):e29828]	PubMed: 38699732
RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons [ Sci Adv, 2023, 9(49):eadi6681]	PubMed: 38055811
Polyubiquitinated PCNA promotes alternative lengthening of telomeres by inducing break-induced replication [ bioRxiv, 2023, 10.1101/2023.07.13.548953]	PubMed: None
G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells [ Nucleic Acids Res, 2021, gkab500]	PubMed: 34139015
Differential responses of neurons, astrocytes, and microglia to G-quadruplex stabilization [ Aging (Albany NY), 2021, 13(12):15917-15941]	PubMed: 34139671

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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