受注:045-509-1970 |
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Synonyms | RR82 Trifluoroacetate Salt | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C31H32N8O5·C2HF3O2 |
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分子量 | 710.7 | CAS No. | 1472611-44-1 | |
Solubility (25°C)* | 体外 | DMSO | 100 mg/mL (140.7 mM) | |
Water | 100 mg/mL (140.7 mM) | |||
Ethanol | 30 mg/mL (42.21 mM) | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Pyridostatin Trifluoroacetate Salt is a G-quadruplexe stabilizer with Kd of 490 nM in a cell-free assay, which targets a series of proto-oncogenes. |
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in vitro | Pyridostatin decreases the proliferation of MRC-5–SV40 cells and various cancer cell lines, and induces cell-cycle arrest by DNA-damage checkpoint activation. Pyridostatin also reduces SRC-dependent cell motility in MDA-MB-231 cells by interacting with G-quadruplex motifs in SRC. [2] Pyridostatin decreases EBNA1 synthesis via inhibition of G-quadruplexes. [3] |
in vivo |
キナーゼアッセイ | ||
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細胞アッセイ | 細胞株 | MRC-5–SV40 cells and various cancer cell lines |
濃度 | 10 μM | |
反応時間 | 48 h | |
実験の流れ | Cells are plated at equal confluence and are left either untreated or were treated with 2 μM pyridostatin continually during the indicated time before harvesting the cells. Cells from individual plates are trypsinized and counted in a Coulter counter. Graphs represent the total cell numbers at each time interval, and the error bars represent the s.e.m. Data represent three independent experiments. |
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動物実験 | 動物モデル | CB17‐SCID mice |
投薬量 | 7.5 mg/kg | |
投与方法 | i.v. |
RAD54L2-mediated DNA damage avoidance pathway specifically preserves genome integrity in response to topoisomerase 2 poisons [ Sci Adv, 2023, 9(49):eadi6681] | PubMed: 38055811 |
G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells [ Nucleic Acids Res, 2021, gkab500] | PubMed: 34139015 |
Differential responses of neurons, astrocytes, and microglia to G-quadruplex stabilization [ Aging (Albany NY), 2021, 13(12):15917-15941] | PubMed: 34139671 |
Characterization of an active LINE-1 in the naked mole-rat genome [ Sci Rep, 2021, 11(1):5725] | PubMed: 33707548 |
hnRNP H/F Drive RNA G-quadruplex-mediated Translation Linked to Genomic Instability and Therapy Resistance in Glioblastoma [ Nat Commun, 2020, 27;11(1):2661] | PubMed: 32461552 |
Replication Stress Induces ATR/CHK1-Dependent Nonrandom Segregation of Damaged Chromosomes. [ Mol Cell, 2020, 22 pii: S1097-2765(20)30231-8] | PubMed: 32353258 |
Regulation of Autophagy by DNA G-quadruplexes [ Autophagy, 2020, 18] | PubMed: 32420812 |
HNRNPH1-dependent splicing of a fusion oncogene reveals a targetable RNA G-quadruplex interaction [ RNA, 2019, 25(12):1731-1750] | PubMed: 31511320 |
HNRNPH1-dependent splicing of a fusion oncogene reveals a targetable RNA G-quadruplex interaction [ RNA, 2019, 25(12):1731-1750] | PubMed: 31511320 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。