Pyrroloquinoline quinone

製品コードS3578 バッチS357801

化学情報

	Synonyms	PQQ, Methoxatin	Storage (From the date of receipt)	3 years -20°C powder
	化学式	C₁₄H₆N₂O₈
	分子量	330.21	CAS No.	72909-34-3
Solubility (25°C)*	体外	DMSO	Insoluble
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Pyrroloquinoline quinone (PQQ, メトキサチン) は、抗酸化作用と抗老化作用を持つ天然の抗酸化物質です。Pyrroloquinoline quinoneはエストロゲン欠乏誘発性骨粗鬆症を予防します。Pyrroloquinoline quinoneは、免疫微小環境を改善し、JAK-STATシグナル伝達経路を調節することにより、マウスのアレルギー性気道炎症を軽減できます。本製品は溶解度が低く、動物実験は可能ですが、細胞実験は慎重にご選択ください！
in vitro	The activation of AMPK by this compound is required for the regulation of mitochondrial biogenesis in rotenone-injured SH-SY5Y cells, the upregulation of PGC-1α induced by this chemical is reduced by inhibition of AMPK.
in vivo	Pyrroloquinoline quinone dose-dependently alleviates the locomotor deficits and nigral dopaminergic neuron loss in PD mice, significantly diminishes the reduction of mitochondria number and their pathological change in the midbrain, also dose-dependently blocks rotenone-caused reduction in the expression of PGC-1α and TFAM, two key activators of mitochondrial gene transcription, in the midbrain.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	SH-SY5Y human neuroblastoma cells
	濃度	100 μM
	反応時間	30 min
	実験の流れ	SH-SY5Y human neuroblastoma cells were cultured in DMEM supplemented with 10% FBS at 37 °C in a humidified atmosphere of 95% air and 5% CO2. The day before treatment, cells were plated in a 10-cm culture dish at a density of 1 × 106 cells/mL. The cells were divided into four groups: the control group (no rotenone or PQQ), this compound group (100 μM PQQ), Rotenone group (100 μM rotenone), and this compound + Rotenone group (100 μM PQQ + 100 μM rotenone). Thirty minutes after treatment, the cells were washed once with cold PBS and scraped in urea lysis buffer (9 M sequanal-grade urea, 20 mM HEPES [pH 8.0], 1 mM β-glyceropho_x005f?sphate, 1 mM sodium vanadate, 2.5 mM sodium pyrophosphate). The cells were sonicated in lysis buffer and centrifuged for 15 min at 4 °C and 20,000 × g. Supernatants were collected and reduced with 4.5 mM DTT for 30 min at 55 °C. Reduced lysates were alkylated with iodoacetamide (0.019 g/mL of H2O) for 15 min in the dark. The samples were diluted 1:4 with 20 mM HEPES [pH 8.0] and digested with 10 g/mL trypsin-tosylphenylalanyl chloromethyl ketone in 1 mM HCl overnight. Digested peptide lysates were acidified with 1% TFA, and the peptides were desalted over 360- mg SEP PAK Classic C18 columns. Peptides were eluted with 40% acetonitrile in 0.1% TFA, dried under a vacuum, and stored at −80 °C. Differentially expressed phosphoproteins were identified and analyzed.
動物実験	動物モデル	Adult ICR mice of PD model
	投薬量	0.8, 4, and 20 mg/kg/d
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/30662347/
https://pubmed.ncbi.nlm.nih.gov/32860006/
https://pubmed.ncbi.nlm.nih.gov/36345503/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Pyrroloquinoline Quinone Reprograms the Single-Cell Landscape of Immune Aging in Hematopoietic Immune System [ Aging Cell, 2025, e70050.]	PubMed: 40192736

Pyrroloquinoline Quinone Reprograms the Single-Cell Landscape of Immune Aging in Hematopoietic Immune System [ Aging Cell, 2025, e70050.]

PubMed: 40192736

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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