Quinidine hemisulfate hydrate

製品コードS3751 バッチS375101

印刷

化学情報

Chemical Structure Synonyms Chinidin Sodium, Pitayine Sodium, β-quinine Sodium, (+)-quinidine Sodium Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C20H24N2O2.1/2H2SO4.H2O
分子量 391.47 CAS No. 6591-63-5
Solubility (25°C)* 体外 DMSO 100 mg/mL (255.44 mM)
Ethanol 100 mg/mL (255.44 mM)
Water 9 mg/mL (22.99 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Quinidine hemisulfate hydrate(Chinidin, Pitayine, β-quinine, (+)-quinidine) acts as a blocker of voltage-gated sodium channels, also an antimuscarinic and antimalarial.
in vitro

Quinidine selectively reduces the proliferation of merlin-deficient HMM cell lines by causing a G0/G1 arrest, whereas the proliferation rates of merlinexpressing HMM cell lines remain unchanged. In addtion to its function as a channel blocker, quinidine is an inhibitor of a specific isoform of the cytochrome P450 family (CYP2D6), which is involved in the detoxification and metabolism of multiple commonly prescribed drugs as well as numerous endogenous substrates[1].
in vivo

Quinidine is a potent and selective inhibitor of P450db and that quinidine can inhibit the in vivo metabolism of debrisoquine in humans. Quinidine is an inhibitor of amphetamine metabolism in vivo[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 HMM cell lines HIB, and TRA
濃度 2.5, 5, 10, 25 μM
反応時間 4 days
実験の流れ

Cells were cultured on T25 cm2 flasks in the presence of different concentrations of quinidine for 4 days. Bromodeoxyuridine was added for 8.5 hours, after which cells were trypsinized and spun down for 10 minutes at 1500 revolutions per minute. Cells were washed twice in 1% BSA/phosphate-buffered saline (PBS) and fixed with 70% ethanol on ice. DNA was denatured by 2N HCl/Triton X100 at room temperature for 30 minutes and sodium tetraborate was added to neutralize the pH level. After centrifugation, cells were resuspended in 1% BSA/0.5% Tween 20/PBS and the cell concentration was adjusted to 1 x 106 cells per test.
動物実験 動物モデル Lewis rats
投薬量 80 mg/kg
投与方法 p.o.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Pathogenesis and drug response of iPSC-derived cardiomyocytes from two Brugada syndrome patients with different Na v1.5-subunit mutations [ J Biomed Res, 2021, 35(5):395-407] PubMed: 34628405
Quantifying drug-induced structural toxicity in hepatocytes and cardiomyocytes derived from hiPSCs using a deep learning method [ J Pharmacol Toxicol Methods, 2020, 106895] PubMed: 32629158

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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