RGD (Arg-Gly-Asp) Peptides

製品コードS8008 バッチS800803

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₂H₂₂N₆O₆
	分子量	346.34	CAS No.	99896-85-2
Solubility (25°C)*	体外	Water	22 mg/mL (63.52 mM)
		DMSO	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	RGD (Arg-Gly-Asp) Peptides is a cell adhesion motif which can mimic cell adhesion proteins and bind to integrins.
in vitro	The RGD sequence is the cell attachment site of a large number of adhesive extracellular matrix, blood, and cell surface proteins, and nearly half of the over 20 known integrins recognize this sequence in their adhesion protein ligands. The RGD peptides and mimics can be used to probe integrin functions in various biological systems. Drug design based on the RGD structure may provide new treatments for diseases such as thrombosis, osteoporosis, and cancer. ^[1] RGD peptide acts as an inhibitor of integrin-ligand interactions and can induce apoptosis in the absence of signals and integrin-mediated cell clustering. Research demonstrates that RGD peptides promote apoptosis through activation of conformation changes that enhance pro-caspase-3 activation and autoprocessing. ^[2] The RGD peptide can serve as a cell adhesion site of extracellular matrix, cell surface proteins, and integrins. ^[3] In addition, RGD peptide can inhibit ACK-2 activation through cell adhesion. ^[4]

製品説明

RGD (Arg-Gly-Asp) Peptides is a cell adhesion motif which can mimic cell adhesion proteins and bind to integrins.

in vitro

The RGD sequence is the cell attachment site of a large number of adhesive extracellular matrix, blood, and cell surface proteins, and nearly half of the over 20 known integrins recognize this sequence in their adhesion protein ligands. The RGD peptides and mimics can be used to probe integrin functions in various biological systems. Drug design based on the RGD structure may provide new treatments for diseases such as thrombosis, osteoporosis, and cancer. ^[1] RGD peptide acts as an inhibitor of integrin-ligand interactions and can induce apoptosis in the absence of signals and integrin-mediated cell clustering. Research demonstrates that RGD peptides promote apoptosis through activation of conformation changes that enhance pro-caspase-3 activation and autoprocessing. ^[2] The RGD peptide can serve as a cell adhesion site of extracellular matrix, cell surface proteins, and integrins. ^[3] In addition, RGD peptide can inhibit ACK-2 activation through cell adhesion. ^[4]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	HLE cell line
	濃度	0.1, 0.3, 1.0 and 2.0 mg/ml
	反応時間	90 min
	実験の流れ	The cells were seeded at approximately 2.6 × 10⁴ /well on each ECM-coated 24-well plate in the medium containing the monotype of GRGDSP RGD peptide at 0 (control), 0.1, 0.3, 1.0 and 2.0 mg/ml. After 90 min of incubation, the floating cells were removed by washing twice with Ca 2+ -Mg 2+ -free phosphate-buffered saline. Then the attached cells were dissociated with trypsin-EDTA solution. The number of cells attached at each concentration of peptide was counted using a hemocytometer.

参考

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カスタマーフィードバック

: Data from [Data independently produced by , , Int J Nanomedicine, 2018, 13:7657-7667]

: Data from [Data independently produced by , , J Biol Chem, 2016, 291(28):14815-25.]

: Data from [Data independently produced by , , Neuroscience, 2019, 396:187-199]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

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