Rhamnose

製品コードE0245 バッチE024501

印刷

化学情報

Chemical Structure Synonyms L-Rhamnose Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C6H12O5
分子量 164.16 CAS No. 3615-41-6
Solubility (25°C)* 体外 DMSO 33 mg/mL (201.02 mM)
 
 
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Rhamnose(L-Rhamnose) is an important 6-deoxy sugar present in many natural products, glycoproteins, and structural polysaccharides.
in vitro

The polymeric copies of a rhamnose motif form glycopolymers (ARGPs), which can bind endogenous antirhamnose antibodies present in human serum, thus attract antibodies from human serum to the target cell surface.[1]
in vivo

Rhamnose(Rha) conjugated immunogens induces high titers of anti-Rha antibodies in wildtype mice, which can replace α1,3galactosyltransferase knockout (α1,3GT KO) mice in such antigen/antibody-mediated vaccine design when developing cancer immunotherapies.[2]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Jurkat T Cells
濃度 1 mg/mL
反応時間 1 h
実験の流れ

Jurkat T cells are resuspended in the RPMI medium without FBS at a final concentration of 350000 cells/mL and placed on ice. After 30 min of incubation on ice, 1 mL of cold cell suspension is incubated for 1 h on ice in the dark with 40 μL of a 1 mg/mL stock solution of this compound in PBS. As a negative control, 1 mL of cold cell suspension is incubated for 1 h on ice in the dark with 40 μL of a 1 mg/mL stock solution of the CholA-p(GalEAmAIBN) polymer in PBS. After the incubation, cells are washed twice with PBS by centrifugation for 3 min at 500 G. Afterward, cells are resuspended in 500 μL of flow incubation buffer (1% bovine serum albumin, 5 mM Na2EDTA in PBS) containing 10% of human serum and are then incubated for 30 min in the dark on ice. After this incubation period, cells are centrifuged at 500 G for 3 min and washed once with PBS. Cells are spun down again at 500 G for 3 min and incubated during 1 h in the dark at room temperature with 500 μL of goat antihuman IgG secondary antibody bound to Alexa 647 at a final concentration of 4 μL/mL and 1% human albumin serum in PBS. Incubation is followed by two washing steps with PBS by centrifugation at 500 G for 5 min. Cells are imaged using stochastic optical reconstruction microscopy.
動物実験 動物モデル BALB/c mice
投薬量 30 µg
投与方法 s.c.

参考

  • https://pubmed.ncbi.nlm.nih.gov/31829561/
  • https://pubmed.ncbi.nlm.nih.gov/21043478/

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。