Rotenone

製品コードS2348 バッチS234803

印刷

化学情報

Synonyms

Barbasco, Dactinol, Paraderil, Rotenon, Rotocide

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₃H₂₂O₆

分子量

394.42

CAS No.

83-79-4

Solubility (25°C)*

体外

DMSO

55 mg/mL (139.44 mM)

Ethanol

2 mg/mL (5.07 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Rotenone is a botanical insecticide that is an inhibitor of mitochondrial electron transport. Rotenone inhibits NADH/DB oxidoreductase and NADH oxidase with IC50 of 28.8 nM and 5.1 nM, respectively. Rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.Rotenone can be used to induce animal models of Parkinson's Disease.
in vitro	This compound inhibits NADH/DB oxidoreductase and NADH oxidase, and induces apoptosis through enhancing mitochondrial reactive oxygen species production.^[1]
in vivo	This mitochondrial toxin, a mitochondrial toxin, induce the up-regulation of alpha-synuclein (alpha-syn) in PD models.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Naïve CD8+ T cells
	濃度	0.1 µM
	反応時間	1 day
	実験の流れ	OCR of CD8+ T cells are analyzed under basal conditions and after sequential injections of 1 μM oligomycin A, 2 μM carbonyl cyanide p-trifluoromethoxyphenylhydrazone, and 0.1 μM rotenone with 1 μM antimycin A. ECAR is analyzed under basal conditions and after sequential injections of 10 mM glucose, 1 μM oligomycin A, and 50 mM 2-deoxyglucose.
動物実験	動物モデル	mice
	投薬量	i.g.
	投与方法

参考

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8641941/
https://pubmed.ncbi.nlm.nih.gov/34531546/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Mitochondrial-cytochrome c oxidase II promotes glutaminolysis to sustain tumor cell survival upon glucose deprivation [ Nat Commun, 2025, 16(1):212]	PubMed: 39747079
Inhibition of PTP1B Through NADH Dehydrogenase Activity Reduces Acute Pancreatic Acinar Cell Pyroptosis by Inhibiting the ROS/TXNIP/NLRP3 Pathway [ Dig Dis Sci, 2025, 10.1007/s10620-025-09412-1]	PubMed: 41051643
Tumor mitochondrial oxidative phosphorylation stimulated by the nuclear receptor RORγ represents an effective therapeutic opportunity in osteosarcoma [ Cell Rep Med, 2024, 5(5):101519]	PubMed: 38692271
SPTBN2 suppresses ferroptosis in NSCLC cells by facilitating SLC7A11 membrane trafficking and localization [ Redox Biol, 2024, 70:103039]	PubMed: 38241838
Knockdown of hepatic mitochondrial calcium uniporter mitigates MASH and fibrosis in mice [ Cell Biosci, 2024, 14(1):135]	PubMed: 39523398
Novel therapeutic strategies targeting bypass pathways and mitochondrial dysfunction to combat resistance to RET inhibitors in NSCLC [ Biochim Biophys Acta Mol Basis Dis, 2024, 1870(6):167249]	PubMed: 38768929
Identifying the potential genes in alpha synuclein driving ferroptosis of Parkinson's disease [ Sci Rep, 2023, 13(1):16893]	PubMed: 37803093
Ginsenoside Rg1 ameliorates chronic intermittent hypoxia-induced vascular endothelial dysfunction by suppressing the formation of mitochondrial reactive oxygen species through the calpain-1 pathway [ J Ginseng Res, 2023, 47(1):144-154]	PubMed: 36644390
Copper induces cell death by targeting lipoylated TCA cycle proteins [ Science, 2022, 375(6586):1254-1261]	PubMed: 35298263
Novel roles of RTN4 and CLIMP-63 in regulating mitochondrial structure, bioenergetics and apoptosis [ Cell Death Dis, 2022, 13(5):436]	PubMed: 35508606

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。