受注:045-509-1970 |
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Synonyms | Mallotoxin, NSC 56346, NSC 94525 | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C30H28O8 |
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分子量 | 516.54 | CAS No. | 82-08-6 | |
Solubility (25°C)* | 体外 | DMSO | 11 mg/mL (21.29 mM) | |
Ethanol | 1 mg/mL (1.93 mM) | |||
Water | ˂1 mg/mL | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | Rottlerin (Mallotoxin, NSC 56346, NSC 94525), a natural compound purified from Mallotus Philippinensis, is a specific Protein kinase inhibitor with IC50 of 3 μM, 6 μM and 5.3 μM for PKCδ(from baculovirus-infected Sf9 insect cells), PKCδ(from porcine spleen) and CaM kinase III, respectively. Rottlerin also inhibits PKCα, PKCγ, PKCβ, PKCη, CKII and PKA with IC50 of 30 μM, 40 μM, 42 μM, 82 μM, 30 μM and 78 μM, respectively. |
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in vitro | Rottlerin is extracted from the plasma and tissues using protein precipitation-extraction and analyzed by reverse-phase HPLC-DAD method. Significant levels of rottlerin are detected in cell lysates from rottlerin-treated HPAF-II cells. These data indicate that rottlerin is efficiently absorbed in cells in vitro.[2] |
in vivo | A xenograft model of pancreatic cancer is prepared by injection of 2×106 HPAF-II cells subcutaneously into nude mice. A substantial amount of Rottlerin is detected in tumor and plasma in mice fed Rottlerin diet. These data indicate that Rottlerin is efficiently absorbed in vivo and suggest a strong potential for Rottlerin as a preventive or adjuvant supplement for pancreatic cancer.[2] |
細胞アッセイ | 細胞株 | HPAF-II cells |
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濃度 | 25 μM | |
反応時間 | 3 h, 24 h | |
実験の流れ | The accumulation of Rottlerin is examined in HPAF-II cells treated with 25 μM Rottlerin, and the fate of Rottlerin in the incubation media. Cells are collected at 3 and 24 h after Rottlerin stimulation and conditioned media are taken at 0.5, 1.0, 3.0, 6.0 and 24 h (n=4 for each time point). Samples are prepared immediately for HPLC analysis without further storage. |
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動物実験 | 動物モデル | nude mice injected with HPAF-II cells |
投薬量 | 120 mg/kg | |
投与方法 | Oral gavage |
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長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。