RS-1

製品コードS8234 バッチS823401

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₀H₁₆Br₂N₂O₃S

分子量

524.23

CAS No.

312756-74-4

Solubility (25°C)*

体外

DMSO

100 mg/mL (190.75 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	2.500mg/ml (4.77mM)	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.800mg/ml (1.53mM)	Taking the 1 mL working solution as an example, add 50 μL of 16 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	RS-1はRAD51刺激化合物であり、RAD51のDNA結合活性を増加させます。これはHDR（相同組換え修復）エンハンサーです。
in vitro	RS-1 stimulates the binding of hRAD51 to ssDNA. Low-micromolar concentrations of this compound enhance DNA binding and result in longer protein–DNA complex lengths. In addition, this small molecule stabilizes the active form of hRAD51 filaments and this is reflected in an enhanced strand assimilation activity. It enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.

製品説明

RS-1はRAD51刺激化合物であり、RAD51のDNA結合活性を増加させます。これはHDR（相同組換え修復）エンハンサーです。

in vitro

RS-1 stimulates the binding of hRAD51 to ssDNA. Low-micromolar concentrations of this compound enhance DNA binding and result in longer protein–DNA complex lengths. In addition, this small molecule stabilizes the active form of hRAD51 filaments and this is reflected in an enhanced strand assimilation activity. It enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Early-passage neonatal human dermal fibroblasts
	濃度	1-7.5 μM
	反応時間	24 h
	実験の流れ	Early-passage neonatal human dermal fibroblasts were maintained in Medium 106 supplemented with low serum growth supplement. Cell-survival experiments were performed using a sulforhodamine B assay. Sulforhodamine B-stained cells are quantified with a Synergy plate reader.

参考

https://pubmed.ncbi.nlm.nih.gov/18840682/
https://pubmed.ncbi.nlm.nih.gov/26817820/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Insufficient telomeric DNA damage response promotes chromosomal instability in aged oocytes [ Sci Bull (Beijing), 2025, S2095-9273(25)00865-5]	PubMed: 40930920
Validation of caprine H11 and the Rosa26 platform for transgene integration via CRISPR-based system: investigations on stable transgene expression and genetic biosafety [ Funct Integr Genomics, 2025, 25(1):180]	PubMed: 40879839
Dual inhibition of SUMOylation and MEK conquers MYC-expressing KRAS-mutant cancers by accumulating DNA damage [ J Biomed Sci, 2024, 31(1):68]	PubMed: 38992694
Generation of VEGF knock-in Cashmere goat via the CRISPR/Cas9 system [ Int J Biol Sci, 2021, 17(4):1026-1040]	PubMed: 33867826
Construction of Synthetic Nanobody Library in Mammalian Cells by dsDNA-Based Strategies* [ Chembiochem, 2021, 22(20):2957-2965]	PubMed: 34411391

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。