RSL3

製品コードS8155 バッチS815505

印刷

化学情報

Synonyms

(1S,3R)-RSL3

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₃H₂₁ClN₂O₅

分子量

440.88

CAS No.

1219810-16-8

Solubility (25°C)*

体外

DMSO

400 mg/mL (907.27 mM)

Ethanol

15 mg/mL (34.02 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	4.000mg/ml (9.07mM)	Taking the 1 mL working solution as an example, add 50 μL of 80 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.250mg/ml (0.57mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	RSL3 ((1S,3R)-RSL3) is a ferroptosis activator in a VDAC-independent manner,exhibiting selectivity for tumor cells bearing oncogenic RAS. RSL3 binds, inactivates GPX4 and thus mediates GPX4-regulated ferroptosis.
in vitro	Ferroptosis-inducing compounds inactivate GPX4 by direct binding or by depleting glutathione.After binding to GPX4, this compound inactivates GPX4 to induce ROS production from lipid peroxidation. Its ability to induce synthetic lethality with oncogenic RAS is rapid and quite potent. This compound inhibits the growth of BJ-TERT/LT/ST/RASV12 and DRD cells as low as 10 ng/mland started to kill sensitive cells as early as 8 hr after treatment^[1]^[2].
in vivo	Prostaglandin-endoperoxide synthase (PTGS) is the key enzyme in prostaglandin biosynthesis. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2. PTGS2 encoding cyclooxygenase-2 (COX-2) is significantly upregulated after treatment with RSL3 and this compound in mice^[1].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	TERT/LT/ST/RASV12 cells
	濃度	1 µg/ml
	反応時間	16hrs
	実験の流れ	TERT/LT/ST/RASV12 cells are seeded in 10 cm dishes and treated with 1 µM staurosporine, 10 µg/ml erastin, 20 µg/ml RSL5, and 1 µg/ml RSL3 for 16 hr. Both dying cells and live cells in each 10 cm dish are harvested and collected in the same 15 ml tubes by centrifuging cell suspension at 1000 rpm for 5 min.
動物実験	動物モデル	athymic nude mice(8 weeks)implanted with subcutaneous xenograft tumors derived from BJeLR cells
	投薬量	100 mg/kg
	投与方法	s.c. injection

参考

https://pubmed.ncbi.nlm.nih.gov/18355723/
https://pubmed.ncbi.nlm.nih.gov/26794443/

カスタマーフィードバック

: , , Am J Physiol Renal Physiol, 2017, doi: 10.1152/ajprenal.00044.2017

: Data from [Data independently produced by , , Nature, 2017, 547(7664):453-457]

: Data from [Data independently produced by , , Cell, 2017, 171(3):628-641]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The Role of Compartmentalized Metabolism in Cellular Metal Homeostasis [ Rockefeller University Digital Commons @ RU, 221, 670]	PubMed: None
NINJ1 regulates plasma membrane fragility under mechanical strain [ Nature, 2025, 10.1038/s41586-025-09222-5]	PubMed: 40490006
Multiple oestradiol functions inhibit ferroptosis and acute kidney injury [ Nature, 2025, 10.1038/s41586-025-09389-x]	PubMed: 40804518
Endoplasmic reticulum-mitochondria contacts are prime hotspots of phospholipid peroxidation driving ferroptosis [ Nat Cell Biol, 2025, 27(6):902-917]	PubMed: 40514428
Autophagy regulator ATG5 preserves cerebellar function by safeguarding its glycolytic activity [ Nat Metab, 2025, 7(2):297-320]	PubMed: 39815080
BDH2-driven lysosome-to-mitochondria iron transfer shapes ferroptosis vulnerability of the melanoma cell states [ Nat Metab, 2025, 7(9):1851-1870]	PubMed: 40957995
Regulation of enzymatic lipid peroxidation in osteoblasts protects against postmenopausal osteoporosis [ Nat Commun, 2025, 16(1):758]	PubMed: 39824794
S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism [ Nat Commun, 2025, 16(1):509]	PubMed: 39779666
Ferroptosis-activating metabolite acrolein antagonizes necroptosis and anti-cancer therapeutics [ Nat Commun, 2025, 16(1):4919]	PubMed: 40425585
Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma [ Cancer Commun (Lond), 2025, 10.1002/cac2.70036]	PubMed: 40443016

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。