受注:045-509-1970 |
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Synonyms | RU320521 | Storage (From the date of receipt) |
3 years -20°C powder | ||||||||
化学式 | C19H12Cl2N4O3 |
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分子量 | 415.23 | CAS No. | 2262452-06-0 | ||||||||
Solubility (25°C)* | 体外 | DMSO | 83 mg/mL (199.88 mM) | ||||||||
Ethanol | 2 mg/mL (4.81 mM) | ||||||||||
Water | Insoluble | ||||||||||
体内 (毎回新しく調製した物を用意してください) |
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* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | RU.521 (RU320521) is an active, potent and selective inhibitor of cyclic GMP-AMP synthase (cGAS) that inhibits the cGAS-mediated signaling with IC50 of 700 nM. RU.521 binds to the cGAS/dsDNA complex with Kd of 36.2 nM. |
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in vitro | RU.521, a cGAS inhibitor, impares the overexpression of interferon-stimulated genes (ISGs) in response to cytolethal distending toxin (CDT), confirming the crucial role of cGAS-STING axis in CDT-induced type I IFN signaling.[2] |
in vivo | RU.521 alleviated the inflammatory response and alleviated the damage induced by oxidative stress, leading to cardiac protection via increased Sirt3 expression in the hearts of mice with sepsis. |
細胞アッセイ | 細胞株 | Mouse Embryonic Fibroblasts (MEFs) |
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濃度 | 1 μM | |
反応時間 | 3, 7, 40 days | |
実験の流れ | MEFs are exposed with or without STINGi or cGASi to CDT 7.5 ng/ml for the indicated times or to the H153A CDT mutant for 40 days. The mRNA level of the indicated genes is analyzed by RT-qPCR and the IL-6 or TNFα production in the culture supernatant are determined by ELISA. For ELISA assays, a 2 days exposure to LPS 100 ng/ml is used as positive control. |
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動物実験 | 動物モデル | Mice |
投薬量 | 5 mg/kg | |
投与方法 | i.p. |
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Inhibiting autophagy enhanced mitotic catastrophe-mediated anticancer immune responses by regulating the cGAS-STING pathway [ Cancer Lett, 2024, 586:216695] | PubMed: 38325769 |
Luteolin inhibits herpes simplex virus 1 infection by activating cyclic guanosine monophosphate-adenosine monophosphate synthase-mediated antiviral innate immunity [ Phytomedicine, 2023, 120:155020] | PubMed: 37632997 |
Spinal TAOK2 contributes to neuropathic pain via cGAS-STING activation in rats [ iScience, 2023, 26(10):107792] | PubMed: 37720090 |
Spinal TAOK2 contributes to neuropathic pain via cGAS-STING activation in rats [ iScience, 2023, 26(10):107792] | PubMed: 37720090 |
FLASH X-ray spares intestinal crypts from pyroptosis initiated by cGAS-STING activation upon radioimmunotherapy [ Proc Natl Acad Sci U S A, 2022, 119(43):e2208506119] | PubMed: 36256824 |
AKT1 Is Required for a Complete Palbociclib-Induced Senescence Phenotype in BRAF-V600E-Driven Human Melanoma [ Cancers (Basel), 2022, 14(3)572] | PubMed: 35158840 |
Inhibition of cGAS in Paraventricular Nucleus Attenuates Hypertensive Heart Injury Via Regulating Microglial Autophagy [ Mol Neurobiol, 2022, 59(11):7006-7024] | PubMed: 36070120 |
Inhibition of the cGAS-STING Pathway Attenuates Lung Ischemia/Reperfusion Injury via Regulating Endoplasmic Reticulum Stress in Alveolar Epithelial Type II Cells of Rats [ J Inflamm Res, 2022, 15:5103-5119] | PubMed: 36091334 |
Chronic exposure to Cytolethal Distending Toxin (CDT) promotes a cGAS-dependent type I interferon response [ Cell Mol Life Sci, 2021, 10.1007/s00018-021-03902-x] | PubMed: 34308492 |
The cGAS-STING signaling pathway contributes to the inflammatory response and autophagy in Aspergillus fumigatus keratitis [ Exp Eye Res, 2020, S0014-4835(20)30624-2] | PubMed: 33227296 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。