Rucaparib Camsylate

製品コードS5195 バッチS519501

印刷

化学情報

Chemical Structure Synonyms Rubraca, AG014699 Camsylate, PF01367338 Camsylate Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C29H34FN3O5S
分子量 555.66 CAS No. 1859053-21-6
Solubility (25°C)* 体外 DMSO 100 mg/mL (179.96 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Rucaparib (Rubraca, AG014699, PF01367338) Camsylateは、PARPの阻害剤であり、セルフリーアッセイにおいてPARP1に対するKiは1.4 nMであり、他の8つのPARPドメインにも結合親和性を示します。
in vitro

Deletion of ATM does not impact sensitivity to PARP inhibition by rucaparib.

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Human prostate cancer lines DU145, ATM-deficient isogenic DU145 cells
濃度 0-5 µM
反応時間 3-4 days
実験の流れ

Cells were seeded into 24-well plates (2,500–4000 cells/well) and treated with increasing concentrations of drug the following day. Cell viability was assessed after 72–96 hours by adding CellTiter-Glo reagent and measuring luminescence using a plate reader. For clonogenic survival assays, cells were seeded in 6-well plates (500–4000 cells/well) in triplicate. Cells were treated with drug 16 to 18 hours after plating and were allowed to grow for 14 days.

(Data sourced from selleck products)

参考

  • https://pubmed.ncbi.nlm.nih.gov/17363489/
  • https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272301/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Co-Targeting of DTYMK and PARP1 as a Potential Therapeutic Approach in Uveal Melanoma [ Cells, 2024, 13(16)1348] PubMed: 39195238
Vanguard is a Glucose Deprivation-Responsive Long Non-Coding RNA Essential for Chromatin Remodeling-Reliant DNA Repair [ Adv Sci (Weinh), 2022, 9(30):e2201210] PubMed: 36047643
Inhibition of BAD-Ser99 phosphorylation synergizes with PARP inhibition to ablate PTEN-deficient endometrial carcinoma [ Cell Death Dis, 2022, 13(6):558] PubMed: 35725817
Cancer Cells Haploinsufficient for ATM Are Sensitized to PARP Inhibitors by MET Inhibition [ Int J Mol Sci, 2022, 23(10)5770] PubMed: 35628590
Combined inhibition of BADSer99 phosphorylation and PARP ablates models of recurrent ovarian carcinoma [ Commun Med (Lond), 2022, 2:82] PubMed: 35791346
Chemoresistance is mediated by ovarian cancer leader cells in vitro [ J Exp Clin Cancer Res, 2021, 40(1):276] PubMed: 34470672
BKM120 sensitizes glioblastoma to the PARP inhibitor rucaparib by suppressing homologous recombination repair [ Cell Death Dis, 2021, 12(6):546] PubMed: 34039959
Pharmacological Poly (ADP-Ribose) Polymerase Inhibitors Decrease Mycobacterium tuberculosis Survival in Human Macrophages [ Front Immunol, 2021, 12:712021] PubMed: 34899683
Synergistic targeting of BRCA1 mutated breast cancers with PARP and CDK2 inhibition [ NPJ Breast Cancer, 2021, 7(1):111] PubMed: 34465787
Role of pyruvate in maintaining cell viability and energy production under high-glucose conditions [ Sci Rep, 2021, 11(1):18910] PubMed: 34556698

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。