SAH (S-Adenosyl-L-homocysteine)

製品コードS7868 バッチS786801

印刷

化学情報

Chemical Structure Synonyms S-Adenosylhomocysteine, AdoHcy, Formycinylhomocysteine Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C14H20N6O5S
分子量 384.41 CAS No. 979-92-0
Solubility (25°C)* 体外 DMSO 77 mg/mL (200.3 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO Corn oil
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 SAH (S-Adenosyl-L-homocysteine) is an inhibitor for (Methyltransferase Like 3-14) METTL3-METTL14 heterodimer complex (METTL3-14) with IC50 of 0.9 μM.
in vitro

S-Adenosyl-L-homocysteine (SAH) shows strong inhibitory effects on METTL3-14 activity with an IC50 value of 0.9 ± 0.1 μM. While the presence of S-Adenosyl-L-homocysteine has no significant effects on ALKBH5 activity.[1]

プロトコル(参考用のみ)

キナーゼアッセイ METTL3-14 Assay
The methyltransferase activity of METTL3-14 complex is measured using a radioactivity-based assay. In this assay, tritiated S-adenosyl-L-methionine (3H-SAM) and unmethylated N6-adenine (A) ss-RNA are used as substrates. After reaction, the biotinylated ss-RNA (m6A) is captured in a FlashPlate coated with streptavidin/scintillant. The amount of methylated m6A ss-RNA is quantified by scintillation counting. All assays are performed using 20 mM Tris pH 7.5, 1 mM DTT, 0.01% Triton X-100, 40 U of RNaseOUT/100 μL buffer. For determination of kinetic parameters, protein concentrations and reaction time are optimized to obtain linear initial velocities. The METTL3-14 complex concentration was 10 nM. To determine the Km values for SAM and ss-RNA, each is titrated into the assay mixture containing the enzyme at the saturating concentration of the other substrate (0.5 μM of SAM or 200 nM ss-RNA).

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

METTL3 promotes osteoblast ribosome biogenesis and alleviates periodontitis [ Clin Epigenetics, 2024, 16(1):18] PubMed: 38267969
M6A modification promotes blood-brain barrier breakdown during cerebral ischemia/reperfusion injury through increasing matrix metalloproteinase 3 expression [ Heliyon, 2023, 9(6):e16905] PubMed: 37332938
Conjugates of adenosine mimetics and arginine-rich peptides serve as inhibitors and fluorescent probes but not as long-lifetime photoluminescent probes for protein arginine methyltransferases [ J Pept Sci, 2022, e3456.] PubMed: 36208424

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。