Datasheet

生物学的記述

Specificity	S100B Antibody [P12F20] detects endogenous levels of total S100B protein.
Background	S100B belongs to the S100 family of EF-hand calcium-binding proteins and serves primarily as an intracellular regulator and extracellular signaling molecule abundant in astrocytes and oligodendrocytes of the central nervous system. It consists of two EF-hand motifs per monomer that form antiparallel homodimers upon calcium binding, enabling interaction with diverse targets through conformational changes that expose hydrophobic surfaces. S100B modulates cytoskeletal dynamics by binding F-actin and microtubule-associated proteins, promoting stress fiber disassembly and membrane ruffling via inhibition of PKC phosphorylation, while also influencing energy metabolism and Ca2+ homeostasis to regulate astrocyte proliferation, differentiation, and migration. S100B acts in a dose-dependent manner through RAGE receptor ligation, triggering at low physiological levels Ras/MEK/ERK/NF-κB/Bcl-2 signaling for neuroprotection, neurite outgrowth, and astrocyte proliferation, but at high pathological concentrations hyperactivating ERK with ROS overproduction to induce neuronal apoptosis; this biphasic action extends to RAGE/p38MAPK/pAkt/mTOR/HIF1α pathways that upregulate VEGF and NO while suppressing p53 in cancer cells. S100B maintains glial shape and motility critical for blood-brain barrier integrity and synaptic support, positioning it as a key target for studying astrocyte-oligodendrocyte interactions in development and repair where calcium flux dictates cellular responses. Its expression in gray matter protoplasmic astrocytes and white matter oligodendrocytes supports tissue-specific roles in myelination and neuroinflammation modulation. Dysregulation with chronic elevation promotes tumor angiogenesis and invasion in colorectal cancer via proinflammatory NF-κB activation.

Specificity

S100B Antibody [P12F20] detects endogenous levels of total S100B protein.

Background

S100B belongs to the S100 family of EF-hand calcium-binding proteins and serves primarily as an intracellular regulator and extracellular signaling molecule abundant in astrocytes and oligodendrocytes of the central nervous system. It consists of two EF-hand motifs per monomer that form antiparallel homodimers upon calcium binding, enabling interaction with diverse targets through conformational changes that expose hydrophobic surfaces. S100B modulates cytoskeletal dynamics by binding F-actin and microtubule-associated proteins, promoting stress fiber disassembly and membrane ruffling via inhibition of PKC phosphorylation, while also influencing energy metabolism and Ca2+ homeostasis to regulate astrocyte proliferation, differentiation, and migration. S100B acts in a dose-dependent manner through RAGE receptor ligation, triggering at low physiological levels Ras/MEK/ERK/NF-κB/Bcl-2 signaling for neuroprotection, neurite outgrowth, and astrocyte proliferation, but at high pathological concentrations hyperactivating ERK with ROS overproduction to induce neuronal apoptosis; this biphasic action extends to RAGE/p38MAPK/pAkt/mTOR/HIF1α pathways that upregulate VEGF and NO while suppressing p53 in cancer cells. S100B maintains glial shape and motility critical for blood-brain barrier integrity and synaptic support, positioning it as a key target for studying astrocyte-oligodendrocyte interactions in development and repair where calcium flux dictates cellular responses. Its expression in gray matter protoplasmic astrocytes and white matter oligodendrocytes supports tissue-specific roles in myelination and neuroinflammation modulation. Dysregulation with chronic elevation promotes tumor angiogenesis and invasion in colorectal cancer via proinflammatory NF-κB activation.

使用情報

Application

WB, IHC, IF

Dilution

WB	IHC	IF
1:1000	1:100-1:400	1:800-1:1600

Reactivity

Human, Mouse, Rat

Source

Rabbit Monoclonal Antibody

MW

10 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/31266264/
https://pubmed.ncbi.nlm.nih.gov/19147496/

Application Data

WB

Validated by Selleck

Lane 1: SK-MEL-28