SAG (Smoothened Agonist) Hydrochloride

製品コードS7779 バッチS777904

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₈H₂₉Cl₂N₃OS
	分子量	526.52	CAS No.	2095432-58-7
Solubility (25°C)*	体外	DMSO	100 mg/mL (189.92 mM)
		Water	100 mg/mL (189.92 mM)
		Ethanol	100 mg/mL (189.92 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SAG (Smoothened Agonist) HClは1種の細胞浸透性Smoothened (Smo)の作動剤で、Shh-LIGHT2細胞の中でEC50 値は3 nMです。
in vitro	SAG regulates Smo activity by binding directly to the Smo heptahelical bundle. SAG induces Smo-dependent signaling through Gli in a GRK2-dependent way. SAG also (1 nM) induces proliferation of neuronal and glial precursors without affecting the differentiation pattern of newly produced cells.
in vivo	In the adult rat hippocampus, the intracerebroventricular administration of SAG (2.5 nM) significantly increases the number of newly generated cells and extends survival of hippocampal cells. In mice, SAG (20 μg/g, i.p.) effectively prevents GC-induced neonatal cerebellar developmental abnormalities.

製品説明

SAG (Smoothened Agonist) HClは1種の細胞浸透性Smoothened (Smo)の作動剤で、Shh-LIGHT2細胞の中でEC50 値は3 nMです。

in vitro

SAG regulates Smo activity by binding directly to the Smo heptahelical bundle.

SAG induces Smo-dependent signaling through Gli in a GRK2-dependent way.

SAG also (1 nM) induces proliferation of neuronal and glial precursors without affecting the differentiation pattern of newly produced cells.

in vivo

In the adult rat hippocampus, the intracerebroventricular administration of SAG (2.5 nM) significantly increases the number of newly generated cells and extends survival of hippocampal cells.

In mice, SAG (20 μg/g, i.p.) effectively prevents GC-induced neonatal cerebellar developmental abnormalities.

プロトコル(参考用のみ)

キナーゼアッセイ	Cell-Based Assays for Hh Pathway Activation
キナーゼアッセイ	Assays for Hh pathway activation in Shh-LIGHT2 cells, a clonal NIH 3T3 cell line stably incorporating Gli-dependent firefly luciferase and constitutive Renilla luciferase reporters, are conducted. For studies of SAG (a chlorobenzothiophene-containing Hh pathway agonist) and PA-SAG, Shh-LIGHT2 cells are cultured to confluency in 96-well plates and then treated with various concentrations of these compounds in DMEM containing 0.5% (vol/vol) bovine calf serum.
細胞アッセイ	細胞株	Shh-LIGHT2 cells
	濃度	3 nM
	反応時間	30 h
	実験の流れ	Cells were treated with various concentrations of SAG in DMEM containing 0.5% (vol/vol) bovine calf serum.
動物実験	動物モデル	Rat
	投薬量	2.5 nM
	投与方法	Intracerebroventricular administration

参考

https://pubmed.ncbi.nlm.nih.gov/12391318/
https://pubmed.ncbi.nlm.nih.gov/16908539/
https://pubmed.ncbi.nlm.nih.gov/20600593/

https://pubmed.ncbi.nlm.nih.gov/22013124/

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カスタマーフィードバック

: Data from [Data independently produced by , , J Pain Res, 2018, 11:649-659]

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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