SAG Hydrochloride

製品コードS7779 バッチS777904

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₈H₂₉Cl₂N₃OS

分子量

526.52

CAS No.

2095432-58-7

Solubility (25°C)*

体外

DMSO (warmed with 50ºC water bath)

100 mg/mL (189.92 mM)

Water

100 mg/mL (189.92 mM)

Ethanol (warmed with 50ºC water bath)

100 mg/mL (189.92 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SAG (Smoothened Agonist) HClは1種の細胞浸透性Smoothened (Smo)の作動剤で、Shh-LIGHT2細胞の中でEC50 値は3 nMです。
in vitro	SAG regulates Smo activity by binding directly to the Smo heptahelical bundle. ^[1] SAG induces Smo-dependent signaling through Gli in a GRK2-dependent way. ^[2] SAG also (1 nM) induces proliferation of neuronal and glial precursors without affecting the differentiation pattern of newly produced cells. ^[3]
in vivo	In the adult rat hippocampus, the intracerebroventricular administration of SAG (2.5 nM) significantly increases the number of newly generated cells and extends survival of hippocampal cells. ^[3] In mice, SAG (20 μg/g, i.p.) effectively prevents GC-induced neonatal cerebellar developmental abnormalities. ^[4]

製品説明

SAG (Smoothened Agonist) HClは1種の細胞浸透性Smoothened (Smo)の作動剤で、Shh-LIGHT2細胞の中でEC50 値は3 nMです。

in vitro

SAG regulates Smo activity by binding directly to the Smo heptahelical bundle. ^[1]

SAG induces Smo-dependent signaling through Gli in a GRK2-dependent way. ^[2]

SAG also (1 nM) induces proliferation of neuronal and glial precursors without affecting the differentiation pattern of newly produced cells. ^[3]

in vivo

In the adult rat hippocampus, the intracerebroventricular administration of SAG (2.5 nM) significantly increases the number of newly generated cells and extends survival of hippocampal cells. ^[3]

In mice, SAG (20 μg/g, i.p.) effectively prevents GC-induced neonatal cerebellar developmental abnormalities. ^[4]

プロトコル(参考用のみ)

キナーゼアッセイ	Cell-Based Assays for Hh Pathway Activation
キナーゼアッセイ	Assays for Hh pathway activation in Shh-LIGHT2 cells, a clonal NIH 3T3 cell line stably incorporating Gli-dependent firefly luciferase and constitutive Renilla luciferase reporters, are conducted. For studies of SAG (a chlorobenzothiophene-containing Hh pathway agonist) and PA-SAG, Shh-LIGHT2 cells are cultured to confluency in 96-well plates and then treated with various concentrations of these compounds in DMEM containing 0.5% (vol/vol) bovine calf serum.
細胞アッセイ	細胞株	Shh-LIGHT2 cells
	濃度	3 nM
	反応時間	30 h
	実験の流れ	Cells were treated with various concentrations of SAG in DMEM containing 0.5% (vol/vol) bovine calf serum.
動物実験	動物モデル	Rat
	投薬量	2.5 nM
	投与方法	Intracerebroventricular administration

参考

https://pubmed.ncbi.nlm.nih.gov/12391318/
https://pubmed.ncbi.nlm.nih.gov/16908539/
https://pubmed.ncbi.nlm.nih.gov/20600593/

https://pubmed.ncbi.nlm.nih.gov/22013124/

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カスタマーフィードバック

: Data from [Data independently produced by , , J Pain Res, 2018, 11:649-659]

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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