Sarsasapogenin

製品コードS3607 バッチS360701

印刷

化学情報

Chemical Structure Synonyms Parigenin Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C27H44O3
分子量 416.64 CAS No. 126-19-2
Solubility (25°C)* 体外 Ethanol 5 mg/mL (12.0 mM)
DMSO Insoluble
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Sarsasapogenin (SAR, Parigenin) is a steroidal sapogenin. It can provoke the generation of reactive oxygen species and activate unfolded protein response (UPR) signaling pathways. SAR potently inhibits NF-κB and MAPK activation, as well as IRAK1, TAK1, and IκBα phosphorylation in LPS-stimulated macrophages.
in vitro Sarsasapogenin induces an increase in the population of Hela cells in S phase and an obvious accumulation of cells in G2/M phase in a time-dependent manner. It induces apoptosis in HeLa cells via the caspase-dependent mitochondrial apoptotic pathway. Sarsasapogenin induces the activation of ER stress pathway. Sarsasapogenin induces cytotoxic effects in human cervical cancer cells via cell cycle arrest, ROS-mediated mitochondrial pathway and ER stress pathway. Sarsasapogenin induces the activation of UPR, the ER specific stress response at early stage and then activates CHOP, which may contribute to the initiation and augment of mitochondrial membrane permeabilization by dephosphorylation of Akt so as to mediate the apoptotic signals from ER to mitochondria[1]. Sarsasapogenin potently inhibits NF-κB and MAPK activation, as well as IRAK1, TAK1, and IκBα phosphorylation in LPS-stimulated macrophages. Sarsasapogenin inhibits the binding of LPS to macrophage Toll-like receptor 4, as well as polarization of M2 to M1 macrophages[2].
in vivo Oral administration of sarsasapogenin inhibits 2,3,4-trinitrobenzene sulfonic acid (TNBS)-induced colon shortening and myeloperoxidase activity in mice, along with reducing NF-κB activation and interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6 levels, while simultaneously increasing IL-10. It inhibits Th17 cell differentiation in colonic lamina propria, but induces Treg cell differentiation. Sarsasapogenin potently inhibits inflammatory responses in vivo[2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Hela cells
濃度 0-60 μM
反応時間 48 h
実験の流れ

HeLa cells are incubated with sarsasapogenin (0-60 μM) for 48 h. Hoechst 33342-stained nuclei are visualized using confocal laser scanning microscopy.
動物実験 動物モデル C57BL/6 mice
投薬量 5 or 10 mg/kg
投与方法 oral

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

A positive feedback loop between gastric cancer cells and tumor-associated macrophage induces malignancy progression [ J Exp Clin Cancer Res, 2022, 41(1):174] PubMed: 35562774

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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