Astragaloside IV

製品コードS3901 バッチS390102

印刷

化学情報

Chemical Structure Synonyms AST-IV, AS-IV Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C41H68O14
分子量 784.97 CAS No. 84687-43-4
Solubility (25°C)* 体外 DMSO 100 mg/mL (127.39 mM)
Water Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Astragaloside IV (AST-IV, AS-IV) is a bioactive saponin first isolated from the dried plant roots of the genus Astragalus, which is used in traditional Chinese medicine. It has various effect on the cardiovascular, immune, digestive, and nervous systems. AS-IV suppresses activation of p-Akt, p-mTOR, p-NF-κB and p-Erk1/2.
in vitro Astragaloside IV probably alleviates leptin resistance by comprehensive modulation of ObR/STAT3 signaling transduction in neuronal cells[2].
in vivo Administration of astragaloside IV (AS-IV) reduces albuminuria, ameliorates changes in the glomerular and tubular pathology, and decreases urinary NAG, NGAL, and TGF-β1 in db/db mice. AS-IV also attenuates the diabetes-related activation of Akt/mTOR, NFκB, and Erk1/2 signaling pathways without causing any detectable hepatotoxicity. AS-IV lowers blood glucose levels in streptozotocin (STZ)-induced diabetic models. It prevents body weight gain and reduced accumulation of fat tissues in HFD fed mice. Astragaloside IV actively participates in the modulation of energy homeostasis[1][2].

プロトコル(参考用のみ)

細胞アッセイ 細胞株 SH-SY5Y cells
濃度 0, 25, 50 and 100 μM
反応時間 24 h
実験の流れ

Neuronal cell line SH-SY5Y cells are maintained in DMEM/F-12 medium supplemented with 10% fetal bovine serum (FBS) at 37 °C in a humidified incubator supplied with 5% CO2. After seeded in 6-well plate at 5 × 105 cells/ml and cultured without FBS overnight, the cells are treated with ASI (0, 25, 50 and 100 μM) for 24 h. Thereafter, they are harvested with CelLyticTM MT mammalian tissue lysis reagent supplemented with protease and phosphatase inhibitors followed by sonication, and stored at −80℃ for further western blotting analysis.
動物実験 動物モデル Six-weeks old male C57BL/6 mice
投薬量 25 mg/kg/d
投与方法 i.p.

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

SUV39H1 Inhibits Angiogenesis in Limb Ischemia of Mice [ Cell Transplant, 2023, 32:9636897231198167] PubMed: 37811706
SUV39H1 Inhibits Angiogenesis in Limb Ischemia of Mice [ Cell Transplant, 2023, 32:9636897231198167] PubMed: 37811706
Astragaloside IV attenuate MI-induced myocardial fibrosis and cardiac remodeling by inhibiting ROS/caspase-1/GSDMD signaling pathway [ Cell Cycle, 2022, 1-14] PubMed: 35770948
Astragaloside IV and echinacoside benefit neuronal properties via direct effects and through upregulation of SOD1 astrocyte function in vitro [ Naunyn Schmiedebergs Arch Pharmacol, 2020, 10.1007/s00210-020-02022-w] PubMed: 33219470

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。