SBI-477

製品コードS3187 バッチS318701

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
化学式

C24H25N3O6S
分子量 483.54 CAS No. 781628-99-7
Solubility (25°C)* 体外 DMSO 97 mg/mL (200.6 mM)
Water Insoluble
Ethanol Insoluble
体内 （毎回新しく調製した物を用意してください）
Clear solution
5%DMSO Corn oil
5.0mg/ml Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 SBI-477 is an insulin signaling inhibitor that deactivates the transcription factor MondoA, leading to reduced expression of the insulin pathway suppressors thioredoxin-interacting protein (TXNIP) and arrestin domain-containing 4 (ARRDC4). SBI-477 inhibits triacylglyceride (TAG) synthesis and enhances basal glucose uptake in human skeletal myocytes.
in vitro

SBI-477, that coordinately inhibits triacylglyceride (TAG) synthesis and enhances basal glucose uptake in human skeletal myocytes, stimulates insulin signaling by deactivating the transcription factor MondoA, leading to reduced expression of the insulin pathway suppressors thioredoxin-interacting protein (TXNIP) and arrestin domain containing 4 (ARRDC4).[1]

プロトコル(参考用のみ)

細胞アッセイ 細胞株 Primary human skeletal myotubes
濃度 --
反応時間 24 h
実験の流れ

Primary human skeletal myotubes were grown and differentiated in 24-well plates. Cells were   treated with the indicated concentration of SBI-477 for 24 h. Following compound treatment, cells were rinsed three times with PBS and then incubated in 125μM [<sup>3</sup>H]-palmitic acid (60 Ci/mmol) bound to fatty acid free albumin containing 1mM carnitine for 2 hours at 37℃. The cell medium was transferred to a tube containing cold 10% trichloroacetic acid (TCA). The tubes were centrifuged at 8,500 x g for 10 minutes at 4℃. The supernatant was immediately removed, mixed with 6N NaOH, and applied to ion-exchange resin. The eluate was collected, measured by liquid scintillation analyzer and normalized to total protein amount. The amount of cell protein was measured.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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