SC75741

製品コードS7273 バッチS727302

印刷

化学情報

Synonyms

N/A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₉H₂₃N₇O₂S₂

分子量

565.67

CAS No.

913822-46-5

Solubility (25°C)*

体外

DMSO (warmed with 50ºC water bath)

35 mg/mL (61.87 mM)

Water

Insoluble

Ethanol

Insoluble

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.875mg/ml (1.55mM)	Taking the 1 mL working solution as an example, add 50 μL of 17.5 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.437mg/ml (0.77mM)	Taking the 1 mL working solution as an example, add 50 μL of 8.74 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SC75741 is a potent NF-κB inhibitor with EC50 of 200 nM. SC75741 efficiently blocks influenza virus propagation.
in vitro	SC75741 shows immunosuppressive activity by inhibiting human PBMC proliferation with IC50 of 2.2 μM. ^[1] This compound inhibits replication of influenza A and B viruses by inhibiting NF-κB-mediated signalling on a transcriptional level. Moreover, it shows a high barrier for development of resistant virus variants. ^[2]
in vivo	SC75741 (15 mg/kg i.p.) reduces virus replication and cytokine expression in mice lungs after H5N1 influenza virus infection. ^[2]
特徴	NF-κB-selective inhibitor.

プロトコル(参考用のみ)

キナーゼアッセイ	NF-κB reporter gene assay
キナーゼアッセイ	The NF-κB reporter gene assay is prepared with A549-NF-κB-SEAP cell line according to manufacturer’s instructions. In short, A549 cells stably transfected with pNF-κB-SEAP reporter gene plasmid are plated and allowed to attach overnight. The cells are subsequently incubated for 5 h with described compounds at 100, 30, 10, 3, 1, 0.3, 0.1, and 0 μM and then stimulated with 10 ng/ml TNF-α for 22 h. The supernatant of the cell is analyzed for SEAP activity using a chemiluminescent SEAP reporter gene assay.
細胞アッセイ	細胞株	Human PBMCs
	濃度	~50 μM
	反応時間	48 hours
	実験の流れ	Cell viability assay is prepared using a CellTiter-BluTM Cell Viability Assay. For each concentration of the compound four replicates are measured.
動物実験	動物モデル	C57BL/6 mice infected with H5N1 influenza virus
	投薬量	~15 mg/kg
	投与方法	i.p.

参考

https://pubmed.ncbi.nlm.nih.gov/17869512/
https://pubmed.ncbi.nlm.nih.gov/23320394/

カスタマーフィードバック

: Data from [Data independently produced by , , Oncotarget, 2016, 7(32):52281-52293]

: Data from [Data independently produced by , , J Nutr Biochem, 2016, 41:56-64. ]

: Data from [Data independently produced by , , J Nutr Biochem, 2017, 41:56-64]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Complementary approaches define the metabolic features that accompany Richter syndrome transformation [ Cell Mol Life Sci, 2025, 82(1):152]	PubMed: 40204982
Critical role of the β isoform of protein kinase C (PKCβ) in angiotensin II-induced oxidative stress in vascular smooth muscle cells [ Physiol Rep, 2025, 13(20):e70595]	PubMed: 41103077
Tumor necrosis factor receptor 2 promotes endothelial cell-mediated suppression of CD8+ T cells through tuning glycolysis in chemoresistance of breast cancer [ J Transl Med, 2024, 22(1):672]	PubMed: 39033271
Hepatocyte growth factor enhances the ability of dental pulp stem cells to ameliorate atherosclerosis in apolipoprotein E-knockout mice [ World J Stem Cells, 2024, 16(5):575-590]	PubMed: 38817328
Ivermectin Enhances Paclitaxel Efficacy by Overcoming Resistance Through Modulation of ABCB1 in Non-small Cell Lung Cancer [ Anticancer Res, 2024, 44(12):5271-5282]	PubMed: 39626921
Inhibiting the glycerophosphodiesterase EDI3 in ER-HER2+ breast cancer cells resistant to HER2-targeted therapy reduces viability and tumour growth [ J Exp Clin Cancer Res, 2023, 42(1):25]	PubMed: 36670508
A Disintegrin and Metalloproteinase with Thrombospondin Motifs 4 Regulates Pulmonary Vascular Hyperpermeability through Destruction of Glycocalyx in Acute Respiratory Distress Syndrome [ Int J Mol Sci, 2023, 10.3390/ijms242216230]	PubMed: 38003418
Development of a nanoparticle-based immunotherapy targeting PD-L1 and PLK1 for lung cancer treatment [ Nat Commun, 2022, 13(1):4261]	PubMed: 35871223
Development of a nanoparticle-based immunotherapy targeting PD-L1 and PLK1 for lung cancer treatment [ Nat Commun, 2022, 13(1):4261]	PubMed: 35871223
Oocytes could rearrange immunoglobulin production to survive over adverse environmental stimuli [ Front Immunol, 2022, 13:990077]	PubMed: 36405746

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。