SC79

製品コードS7863 バッチS786301

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₁₇H₁₇ClN₂O₅
	分子量	364.78	CAS No.	305834-79-1
Solubility (25°C)*	体外	DMSO	72 mg/mL (197.37 mM)
		Ethanol	72 mg/mL warmed with 50ºC water bath (197.37 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SC79 is a brain-penetrable Akt phosphorylation activator and an inhibitor of Akt-PH domain translocation.
in vitro	SC79 suppresses PH_AKTM-GFP plasma membrane translocation, and enhances phosphorylation of all three Akt isoforms in HEK293, HeLa, HL60, NB4, and HsSulton (B cells) cells. SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. ^[1] SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells. ^[2]
in vivo	In the permanent focal cerebral ischemia mouse model, SC79 (0.04 mg/g, i.p.) enables the cytosolic activation of Akt, and recapitulates the primary cellular function of Akt signaling, resulting in augmented neuronal survival. ^[1]

プロトコル(参考用のみ)

キナーゼアッセイ	Cytosolic phosphorylation of Akt
キナーゼアッセイ	Hela cells are serum starved for 1 hr and treated with IGF (100ng/mL) or SC79 (4 μg/mL) for 30 minutes. Cells are lysed in Lysis buffer containing 250 mM Sucrose, 20 mM HEPES, 10 mM KCl, 1.5 mM MgCl₂, 1 mM EDTA, 1 mM EGTA supplemented with protease inhibitors. Cells are passed through 25G needle several times and kept on ice for 20 minutes. Total cell lysate is taken at this point. Cell lysates are centrifuged at 100,000g for 30 minutes. Supernatant is collected as the cytosolic fraction. Pellet is washed with lysis buffer and represents the membrane fraction. Total cell lysate, cytosolic and membrane fractions are resolved by SDS-PAGE and analyzed for phospho-Akt (S473), Total Akt, Tubulin (cytosolic marker) and Orai1 (membrane marker) by western blotting.
細胞アッセイ	細胞株	HsSultan and NB4 cells
	濃度	8 μg/mL
	反応時間	24 h
	実験の流れ	HsSultan or NB4 cells (2.5 × 10⁵) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 µg/mL) is added and cultured for overnight (16–20 h). Fifty microliters of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1 M HCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.
動物実験	動物モデル	Permanent focal cerebral ischemia mouse model
	投薬量	0.04 mg/g
	投与方法	i.p.

参考

カスタマーフィードバック

: Data from [Data independently produced by , , J Exp Clin Cancer Res, 2018, 37(1):41]

: Data from [Data independently produced by , , J Exp Clin Cancer Res, 2017, 36(1):105]

: Data from [Data independently produced by , , EBioMedicine, 2018, 34:35-45]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Combined inhibition of HER2 and VEGFR synergistically improves therapeutic efficacy via PI3K-AKT pathway in advanced ovarian cancer [ J Exp Clin Cancer Res, 2024, 43(1):56]	PubMed: 38403634
A novel DDIT3 activator dehydroevodiamine effectively inhibits tumor growth and tumor cell stemness in pancreatic cancer [ Phytomedicine, 2024, 155377]	PubMed: none
Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling [ Genes Dis, 2024, 11(2):964-977]	PubMed: 37692479
IFN-γ decreases PD-1 in T lymphocytes from convalescent COVID-19 patients via the AKT/GSK3β signaling pathway [ Sci Rep, 2024, 14(1):5038]	PubMed: 38424104
PHLPP1 inhibits the growth and aerobic glycolysis activity of human ovarian granular cells through inactivating AKT pathway [ BMC Womens Health, 2024, 24(1):25]	PubMed: 38184561
Sulindac selectively induces autophagic apoptosis of GABAergic neurons and alters motor behaviour in zebrafish [ Nat Commun, 2023, 14(1):5351]	PubMed: 37660128
Sulindac selectively induces autophagic apoptosis of GABAergic neurons and alters motor behaviour in zebrafish [ Nat Commun, 2023, 14(1):5351]	PubMed: 37660128
Inhibition of extranodal NK/T-cell lymphoma by Chiauranib through an AIF-dependent pathway and its synergy with L-asparaginase [ Cell Death Dis, 2023, 14(5):316]	PubMed: 37160920
NPM promotes hepatotoxin-induced fibrosis by inhibiting ROS-induced apoptosis of hepatic stellate cells and upregulating lncMIAT-induced TGF-β2 [ Cell Death Dis, 2023, 14(8):575]	PubMed: 37648688
NPM promotes hepatotoxin-induced fibrosis by inhibiting ROS-induced apoptosis of hepatic stellate cells and upregulating lncMIAT-induced TGF-β2 [ Cell Death Dis, 2023, 14(8):575]	PubMed: 37648688

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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