Schisandrin A

製品コードS3822 バッチS382201

印刷

化学情報

Synonyms

Deoxyschizandrin, Wuweizisu A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₄H₃₂O₆

分子量

416.51

CAS No.

61281-38-7

Solubility (25°C)*

体外

DMSO

83 mg/mL (199.27 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Schisandrin A（Sch A、Deoxyschizandrin、Wuweizisu A）は、チョウセンゴミシの活性成分であり、肝保護、抗腫瘍、および抗酸化作用を示します。これは、IC50値3.5 µMのアディポネクチン受容体2（AdipoR2）のアゴニストです。
in vitro	Schisandrin A significantly suppresses the lipopolysaccharide (LPS)-induced production of the key pro-inflammatory mediators nitric oxide (NO) and prostaglandin E2 by suppressing the expression of inducible NO synthase and cyclooxygenase-2 at the mRNA and protein levels in RAW 264.7 macrophages. It is demonstrated to reduce the LPS-induced secretion of pro-inflammatory cytokines, including tumor necrosis factor-α and interleukin-1β; this is accompanied by a simultaneous decrease in the respective mRNA and protein levels in the macrophages. In addition, the LPS- induced translocation of nuclear factor-κB (NF-κB), as well as activation of mitogen-activated protein kinases (MAPKs) and phosphatidylinositol-3 kinase (PI3K)/Akt pathways are inhibited by this compound. These results suggest that this chemical has a protective effect against LPS-induced inflammatory and oxidative responses in RAW 264.7 cells by inhibiting the NF-κB, MAPK and PI3K/Akt pathways. It possesses anti-inflammatory activities and excellent Nrf2-induction or ROS-scavenging abilities. This compound can inhibit the replication of four serotypes of DENV in a concentration- and time-dependent manner, with an effective half-maximal effective concentration 50% (EC50) value of 28.1 ± 0.42 μM against DENV serotype type 2 without significant cytotoxicity.
in vivo	Schisandrin A has proven beneficial in preventing cell damage in the pathogenesis of central nervous system diseases, including ischemia. This compound can effectively protect mice from DENV infection by reducing disease symptoms and mortality of DENV-infected mice. It stimulates IFN-mediated antiviral responses in vivo.

プロトコル(参考用のみ)

細胞アッセイ	細胞株	The RAW 264.7 murine macrophage cell line
	濃度	0-200 μM
	反応時間	1 h
	実験の流れ	To evaluate the cytotoxicity of schisandrin A, RAW 264.7 cells are seeded in 96-well plates at a density of 1×10³ cells/well. The cells are treated with various concentrations of this compound for 1 h prior to incubation with LPS (100 ng/ml) for 24 h. After the incubation was complete, images of cells from each well are captured under a phase-contrast microscope. Subsequently, MTT is added to each well at 0.5 mg/ml, followed by incubation at 37°C in the dark. After 3 h of incubation, the MTT solution is removed and 200 μl 5% DMSO is added to dissolve the crystals. The viable cells are detected by reading the absorbance of formazan at 540 nm using an enzyme-linked immunosorbent assay (ELISA) microplate reader. The optical density of the formazan formed in the control (untreated) cells is considered to represent 100% viability.
動物実験	動物モデル	Male Kunming White mice
	投薬量	1, 10, 20 mg/kg d
	投与方法	intragastrically administrated

参考

https://pubmed.ncbi.nlm.nih.gov/24975096/
https://pubmed.ncbi.nlm.nih.gov/29115385/
https://pubmed.ncbi.nlm.nih.gov/28338050/

https://pubmed.ncbi.nlm.nih.gov/23691032/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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