Schisandrin A

製品コードS3822 バッチS382201

印刷

化学情報

Synonyms

Deoxyschizandrin, Wuweizisu A

Storage
(From the date of receipt)

3 years -20°C powder
1 years -80°C in solvent

化学式

C₂₄H₃₂O₆

分子量

416.51

CAS No.

61281-38-7

Solubility (25°C)*

体外

DMSO

83 mg/mL (199.27 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Schisandrin A (Sch A, Deoxyschizandrin, Wuweizisu A) is an active component of Schisandrae Fructus with liver-protective, antitumor, and antioxidant activities. It is an agonist of the adiponectin receptor 2 (AdipoR2) with the IC50 value of 3.5 μM.
in vitro	Schisandrin A significantly suppresses the lipopolysaccharide (LPS)-induced production of the key pro-inflammatory mediators nitric oxide (NO) and prostaglandin E2 by suppressing the expression of inducible NO synthase and cyclooxygenase-2 at the mRNA and protein levels in RAW 264.7 macrophages. It is demonstrated to reduce the LPS-induced secretion of pro-inflammatory cytokines, including tumor necrosis factor-α and interleukin-1β; this is accompanied by a simultaneous decrease in the respective mRNA and protein levels in the macrophages. In addition, the LPS- induced translocation of nuclear factor-κB (NF-κB), as well as activation of mitogen-activated protein kinases (MAPKs) and phosphatidylinositol-3 kinase (PI3K)/Akt pathways are inhibited by this compound. These results suggest that this chemical has a protective effect against LPS-induced inflammatory and oxidative responses in RAW 264.7 cells by inhibiting the NF-κB, MAPK and PI3K/Akt pathways. It possesses anti-inflammatory activities and excellent Nrf2-induction or ROS-scavenging abilities^[2]. This compound can inhibit the replication of four serotypes of DENV in a concentration- and time-dependent manner, with an effective half-maximal effective concentration 50% (EC50) value of 28.1 ± 0.42 μM against DENV serotype type 2 without significant cytotoxicity^[3].
in vivo	Schisandrin A has proven beneficial in preventing cell damage in the pathogenesis of central nervous system diseases, including ischemia^[2]. This compound can effectively protect mice from DENV infection by reducing disease symptoms and mortality of DENV-infected mice. It stimulates IFN-mediated antiviral responses in vivo^[3].

プロトコル(参考用のみ)

細胞アッセイ	細胞株	The RAW 264.7 murine macrophage cell line
	濃度	0-200 μM
	反応時間	1 h
	実験の流れ	To evaluate the cytotoxicity of schisandrin A, RAW 264.7 cells are seeded in 96-well plates at a density of 1×10³ cells/well. The cells are treated with various concentrations of this compound for 1 h prior to incubation with LPS (100 ng/ml) for 24 h. After the incubation was complete, images of cells from each well are captured under a phase-contrast microscope. Subsequently, MTT is added to each well at 0.5 mg/ml, followed by incubation at 37°C in the dark. After 3 h of incubation, the MTT solution is removed and 200 μl 5% DMSO is added to dissolve the crystals. The viable cells are detected by reading the absorbance of formazan at 540 nm using an enzyme-linked immunosorbent assay (ELISA) microplate reader. The optical density of the formazan formed in the control (untreated) cells is considered to represent 100% viability.
動物実験	動物モデル	Male Kunming White mice
	投薬量	1, 10, 20 mg/kg d
	投与方法	intragastrically administrated

参考

https://pubmed.ncbi.nlm.nih.gov/24975096/
https://pubmed.ncbi.nlm.nih.gov/29115385/
https://pubmed.ncbi.nlm.nih.gov/28338050/

https://pubmed.ncbi.nlm.nih.gov/23691032/

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長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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