SCR7

製品コードS7742 バッチS774202

印刷

化学情報

Chemical Structure Synonyms N/A Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

C18H12N4OS
分子量 332.38 CAS No. 14892-97-8
Solubility (25°C)* 体外 DMSO 66 mg/mL (198.56 mM)
Ethanol 3 mg/mL warmed with 50ºC water bath (9.02 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 SCR7 is a specific DNA Ligase IV inhibitor, which blocks nonhomologous end-joining (NHEJ).
in vitro SCR7 effectively inhibits the formation of multimers at 200 μM and above. SCR7 successfully inhibits cell proliferation of MCF7, A549, HeLa, T47D, A2780, HT1080, and Nalm6 with IC50 of 40, 34, 44, 8.5, 120, 10, and 50 μM, respectively.[1] SCR7 suppresses the NHEJ repair of CRISPR-Cas9-induced DSBs.[2]SCR7 increases the efficiency of HDR-mediated genome editing up to 19-fold using CRISPR/Cas9 in mammalian cells and mouse embryos[3].
in vivo SCR7 treatment (10 mg/kg, i.m.) significantly reduces breast adenocarcinoma-induced tumor, and exhibits 4-fold increase in lifespan compared with control group. However, in Swiss albino mice with Dalton’s lymphoma tumor model, SCR7 (20 mg/kg, i.p.) exhibits neither tumor regression nor increase in lifespan. In BALB/c mice, SCR7 (20 mg/kg, i.p.) significantly enhances the cytotoxic effects of radiation, etoposide and 3-Aminobenzamide on tumor derived from Dalton’s lymphoma (DLA) cells.[1]

プロトコル(参考用のみ)

キナーゼアッセイ Complementation of SCR7 Inhibition with Purified Ligase IV
Complementation experiment is carried out by adding increasing concentrations of purified Ligase IV/XRCC4 complex (30, 60, and 120 fmol) along with the oligomeric DNA substrates (5’ compatible and 5’-5’ noncompatible ends) to the SCR7-treatedextracts. Reactions are incubated for 2 h at 25℃. The reaction products are then resolved on 8% denaturing PAGE. The gel is dried and exposed and the signal is detected with a PhosphorImager and analyzed with Multi Gauge (V3.0) software.
細胞アッセイ 細胞株 MCF7, CEM, HeLa, A549, HT1080, A2780, T47D, Nalm6, N114 and K562 cells
濃度 250 μM
反応時間 48 h
実験の流れ

Cell proliferation of cancer cells are determined by MTT and trypan blue assays. Briefly, MCF7, CEM, HeLa, A549, HT1080, A2780, T47D, Nalm6, N114 and K562 cells are grown in presence of SCR7 (10, 50, 100, and 250 μM) for 24 or 48 h, and subjected to MTT or trypan blue assays. Each experiment is repeated a minimum of three independent times.
動物実験 動物モデル BALB/c mice
投薬量 10 mg/kg
投与方法 i.m.

カスタマーフィードバック

, , J Cell Mol Med, 2017, 21(12):3337-3346

Data from [Data independently produced by , , Cell Res, 2017, 27(6):801-814]

Data from [Data independently produced by , , Mutat Res Genet Toxicol Environ Mutagen, 2015, 793:2-8]

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

The fragile X locus is prone to spontaneous DNA damage that is preferentially repaired by nonhomologous end-joining to preserve genome integrity [ iScience, 2024, 27(2):108814] PubMed: 38303711
Simultaneous inhibition of DNA-PK and Polϴ improves integration efficiency and precision of genome editing [ Nat Commun, 2023, 14(1):4761] PubMed: 37580318
MDM2 antagonists promote CRISPR/Cas9-mediated precise genome editing in sheep primary cells [ Mol Ther Nucleic Acids, 2023, 31:309-323] PubMed: 36726409
Selective utilization of non-homologous end-joining and homologous recombination for DNA repair during meiotic maturation in mouse oocytes [ Cell Prolif, 2023, 56(4):e13384] PubMed: 36564861
Increase of c-FOS promoter transcriptional activity by the dual leucine zipper kinase [ Naunyn Schmiedebergs Arch Pharmacol, 2023, none] PubMed: 36700987
Identifying key underlying regulatory networks and predicting targets of orphan C/D box SNORD116 snoRNAs in Prader-Willi syndrome [ bioRxiv, 2023, 10.1101/2023.10.03.560773] PubMed: 37873184
Disparate pathways for extrachromosomal DNA biogenesis and genomic DNA repair [ bioRxiv, 2023, 2023.10.22.563489] PubMed: 37961138
Functional editing of endogenous genes through rapid selection of cell pools (Rapid generation of endogenously tagged genes in Drosophila ovarian somatic sheath cells) [ Nucleic Acids Res, 2022, gkac448] PubMed: 35639929
Development of a Novel Reference Material for Tumor Mutational Burden Measurement Based on CRISPR/Cas9 Technology [ Front Oncol, 2022, 12:845636] PubMed: 35574377
MCL-1 interacts with MOF and BID to regulate H4K16 acetylation and homologous recombination repair [ Cell Biol Int, 2022, 46(8):1196-1203] PubMed: 35661479

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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