Datasheet

生物学的記述

Specificity	SMG-1 Antibody [L4N6] detects endogenous levels of total SMG-1 protein.
Background	SMG‑1 is a large serine/threonine kinase and a member of the phosphoinositide 3‑kinase‑related kinase (PIKK) family, which includes ATM, ATR, mTOR, DNA‑PKcs, and TRRAP, and it is widely expressed in mammalian cells where it functions as a core regulator of mRNA quality control and stress‑responsive signaling. SMG‑1 forms a stable complex with SMG‑8 and SMG‑9, and this assembly targets the RNA‑binding protein UPF1 (hUpf1) for phosphorylation following recognition of premature termination codons on spliced mRNAs, thereby triggering nonsense‑mediated mRNA decay (NMD) and preventing the accumulation of truncated, nonfunctional or aberrant proteins. SMG‑1 phosphorylates p53 and other substrates in response to DNA damage and genotoxic stress, and it contributes to cell‑cycle control by modulating the G1/S checkpoint through both p53‑dependent and p53‑independent mechanisms, including regulation of Cdc25A and suppression of CDK2 activity. SMG‑1 activity intersects with DNA‑damage and survival pathways, and exhibits the potential to protect cells from extrinsically induced apoptosis by influencing cell‑death‑receptor signaling and by stabilizing anti‑apoptotic regulators such as c‑FLIP, which dampens caspase‑8 activation in response to TNFα and Smac‑mimetic compounds. Loss or inhibition of SMG‑1 increases sensitivity to DNA‑damaging agents and to extrinsic apoptotic stimuli, and reduced SMG‑1 expression or function has been associated with impaired NMD, altered cell‑cycle progression, and either tumor‑suppressive or context‑dependent oncogenic phenotypes in different cancers.

Specificity

SMG-1 Antibody [L4N6] detects endogenous levels of total SMG-1 protein.

Background

SMG‑1 is a large serine/threonine kinase and a member of the phosphoinositide 3‑kinase‑related kinase (PIKK) family, which includes ATM, ATR, mTOR, DNA‑PKcs, and TRRAP, and it is widely expressed in mammalian cells where it functions as a core regulator of mRNA quality control and stress‑responsive signaling. SMG‑1 forms a stable complex with SMG‑8 and SMG‑9, and this assembly targets the RNA‑binding protein UPF1 (hUpf1) for phosphorylation following recognition of premature termination codons on spliced mRNAs, thereby triggering nonsense‑mediated mRNA decay (NMD) and preventing the accumulation of truncated, nonfunctional or aberrant proteins. SMG‑1 phosphorylates p53 and other substrates in response to DNA damage and genotoxic stress, and it contributes to cell‑cycle control by modulating the G1/S checkpoint through both p53‑dependent and p53‑independent mechanisms, including regulation of Cdc25A and suppression of CDK2 activity. SMG‑1 activity intersects with DNA‑damage and survival pathways, and exhibits the potential to protect cells from extrinsically induced apoptosis by influencing cell‑death‑receptor signaling and by stabilizing anti‑apoptotic regulators such as c‑FLIP, which dampens caspase‑8 activation in response to TNFα and Smac‑mimetic compounds. Loss or inhibition of SMG‑1 increases sensitivity to DNA‑damaging agents and to extrinsic apoptotic stimuli, and reduced SMG‑1 expression or function has been associated with impaired NMD, altered cell‑cycle progression, and either tumor‑suppressive or context‑dependent oncogenic phenotypes in different cancers.

使用情報

Application

WB, IP

Dilution

WB	IP
1:1000	1:50

Reactivity

Human, Monkey

Source

Rabbit Monoclonal Antibody

MW

410 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/16289965/
https://pubmed.ncbi.nlm.nih.gov/24107632/

Application Data

WB

Validated by Selleck

Lane 1: U2OS, Lane 2: 293, Lane 3: MCF7