SAG (Smoothened Agonist) HCl

製品コードS7779 バッチS777902

化学情報

	Synonyms	N/A	Storage (From the date of receipt)	3 years -20°C powder 1 years -80°C in solvent
	化学式	C₂₈H₂₉Cl₂N₃OS
	分子量	526.52	CAS No.	2095432-58-7
Solubility (25°C)*	体外	DMSO	100 mg/mL warmed with 50ºC water bath (189.92 mM)
		Water	100 mg/mL (189.92 mM)
		Ethanol	40 mg/mL warmed with 50ºC water bath (75.97 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SAG (Smoothened Agonist) HClは1種の細胞浸透性Smoothened (Smo)の作動剤で、Shh-LIGHT2細胞の中でEC50 値は3 nMです。
in vitro	SAG regulates Smo activity by binding directly to the Smo heptahelical bundle. ^[1] SAG induces Smo-dependent signaling through Gli in a GRK2-dependent way. ^[2] SAG also (1 nM) induces proliferation of neuronal and glial precursors without affecting the differentiation pattern of newly produced cells. ^[3]
in vivo	In the adult rat hippocampus, the intracerebroventricular administration of SAG (2.5 nM) significantly increases the number of newly generated cells and extends survival of hippocampal cells. ^[3] In mice, SAG (20 μg/g, i.p.) effectively prevents GC-induced neonatal cerebellar developmental abnormalities. ^[4]

製品説明

SAG (Smoothened Agonist) HClは1種の細胞浸透性Smoothened (Smo)の作動剤で、Shh-LIGHT2細胞の中でEC50 値は3 nMです。

in vitro

SAG regulates Smo activity by binding directly to the Smo heptahelical bundle. ^[1]

SAG induces Smo-dependent signaling through Gli in a GRK2-dependent way. ^[2]

SAG also (1 nM) induces proliferation of neuronal and glial precursors without affecting the differentiation pattern of newly produced cells. ^[3]

in vivo

In the adult rat hippocampus, the intracerebroventricular administration of SAG (2.5 nM) significantly increases the number of newly generated cells and extends survival of hippocampal cells. ^[3]

In mice, SAG (20 μg/g, i.p.) effectively prevents GC-induced neonatal cerebellar developmental abnormalities. ^[4]

プロトコル(参考用のみ)

キナーゼアッセイ	Cell-Based Assays for Hh Pathway Activation
キナーゼアッセイ	Assays for Hh pathway activation in Shh-LIGHT2 cells, a clonal NIH 3T3 cell line stably incorporating Gli-dependent firefly luciferase and constitutive Renilla luciferase reporters, are conducted. For studies of SAG (a chlorobenzothiophene-containing Hh pathway agonist) and PA-SAG, Shh-LIGHT2 cells are cultured to confluency in 96-well plates and then treated with various concentrations of these compounds in DMEM containing 0.5% (vol/vol) bovine calf serum.
細胞アッセイ	細胞株	Shh-LIGHT2 cells
	濃度	3 nM
	反応時間	30 h
	実験の流れ	Cells were treated with various concentrations of SAG in DMEM containing 0.5% (vol/vol) bovine calf serum.
動物実験	動物モデル	Rat
	投薬量	2.5 nM
	投与方法	Intracerebroventricular administration

参考

[4] Heine VM, et al. Sci Transl Med. 2011, 3(105), 105ra104.

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カスタマーフィードバック

: Data from [Data independently produced by , , J Pain Res, 2018, 11:649-659]

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