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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder 1 years -80°C in solvent |
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化学式 | C28H29Cl2N3OS |
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分子量 | 526.52 | CAS No. | 2095432-58-7 | |
Solubility (25°C)* | 体外 | Water | 100 mg/mL (189.92 mM) | |
DMSO | 56 mg/mL warmed with 50ºC water bath (106.35 mM) | |||
Ethanol | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | SAG (Smoothened Agonist) HClは1種の細胞浸透性Smoothened (Smo)の作動剤で、Shh-LIGHT2細胞の中でEC50 値は3 nMです。 |
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in vitro | SAG regulates Smo activity by binding directly to the Smo heptahelical bundle. [1] SAG induces Smo-dependent signaling through Gli in a GRK2-dependent way. [2] SAG also (1 nM) induces proliferation of neuronal and glial precursors without affecting the differentiation pattern of newly produced cells. [3] |
in vivo | In the adult rat hippocampus, the intracerebroventricular administration of SAG (2.5 nM) significantly increases the number of newly generated cells and extends survival of hippocampal cells. [3] In mice, SAG (20 μg/g, i.p.) effectively prevents GC-induced neonatal cerebellar developmental abnormalities. [4] |
キナーゼアッセイ | Cell-Based Assays for Hh Pathway Activation | |
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Assays for Hh pathway activation in Shh-LIGHT2 cells, a clonal NIH 3T3 cell line stably incorporating Gli-dependent firefly luciferase and constitutive Renilla luciferase reporters, are conducted. For studies of SAG (a chlorobenzothiophene-containing Hh pathway agonist) and PA-SAG, Shh-LIGHT2 cells are cultured to confluency in 96-well plates and then treated with various concentrations of these compounds in DMEM containing 0.5% (vol/vol) bovine calf serum. | ||
細胞アッセイ | 細胞株 | Shh-LIGHT2 cells |
濃度 | 3 nM | |
反応時間 | 30 h | |
実験の流れ | Cells were treated with various concentrations of SAG in DMEM containing 0.5% (vol/vol) bovine calf serum. |
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動物実験 | 動物モデル | Rat |
投薬量 | 2.5 nM | |
投与方法 | Intracerebroventricular administration |
Data from [Data independently produced by , , J Pain Res, 2018, 11:649-659]
Spatially controlled construction of assembloids using bioprinting [ Nat Commun, 2023, 14(1):4346] | PubMed: 37468483 |
Highly reproducible and cost-effective one-pot organoid differentiation using a novel platform based on PF-127 triggered spheroid assembly [ Biofabrication, 2023, 15(4).] | PubMed: 37552975 |
Selective induction of human renal interstitial progenitor-like cell lineages from iPSCs reveals development of mesangial and EPO-producing cells [ Cell Rep, 2023, S2211-1247(23)01614-5] | PubMed: 38237600 |
Acquisition of neural fate by combination of BMP blockade and chromatin modification [ iScience, 2023, 26(10):107887] | PubMed: 37771660 |
Small Molecule Cocktails Promote Fibroblast-to-Leydig-like Cell Conversion for Hypogonadism Therapy [ Pharmaceutics, 2023, 10.3390/pharmaceutics15102456] | PubMed: 37896216 |
Primary cilia support cartilage regeneration after injury [ Int J Oral Sci, 2023, 15(1):22] | PubMed: 37268650 |
Transient agonism of the sonic hedgehog pathway triggers a permanent transition of skin appendage fate in the chicken embryo [ Sci Adv, 2023, 9(20):eadg9619] | PubMed: 37196093 |
Polyhomeotic homolog 1 (Phc1) confers a permissive effect during neural differentiation through epigenetic regulation [ naist, 2023 , ] | PubMed: none |
A PKA inhibitor motif within SMOOTHENED controls Hedgehog signal transduction [ Nat Struct Mol Biol, 2022, 29(10):990-999] | PubMed: 36202993 |
Extended intergenic DNA contributes to neuron-specific expression of neighboring genes in the mammalian nervous system [ Nat Commun, 2022, 13(1):2733] | PubMed: 35585070 |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。