SodiuM Metatungstate

製品コードS5525 バッチS552501

印刷

化学情報

Synonyms

Sodium Polytungstate

Storage
(From the date of receipt)

3 years -20°C powder

化学式

H₂O₄₀W₁₂.6Na

分子量

2986.01

CAS No.

12141-67-2

Solubility (25°C)*

体外

Water

50 mg/mL (16.74 mM)

DMSO

30 mg/mL (10.04 mM)

体内（毎回新しく調製した物を用意してください）

Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	1.5mg/ml (0.50mM)	Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil この製剤はselleckのラボで検証済みです。上記の溶解方法がご要望を満たさない場合、selleckの営業担当までお問い合わせ頂ければ、個別の試験を行います。	0.21mg/ml (0.07mM)	Taking the 1 mL working solution as an example, add 50 μL of 4.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	Sodium metatungstate (SMT) solution is an inorganic heavy liquid which is widely used in density fractionation. Sodium metatungstate (Sodium polyoxotungstates) is a potent and selective inhibitor of ecto-nucleoside triphosphate diphosphohydrolase (NTPDases, CD39) with Ki of 0.140 μM, 0.910 μM and 0.563 μM for NTPDase1, NTPDase2 and NTPDase3, respectively.
in vitro	Sodium metatungstate, a inhibitor of CD39, can partially reverse the suppression of CD8[₊H] T cells induced by the presence of peritoneal macrophage.^[2]

プロトコル(参考用のみ)

細胞アッセイ	細胞株	Peritoneal lavage cells, CD8₊ T cells (purified from ACK-lysed splenocytes from C57BL/6 mice)
	濃度	50 μM
	反応時間	48 h
	実験の流れ	Peritoneal lavage cells are plated in complete RPMI media on a 10 cm dish. Celexocib (10 μM), anti-TGF-b (clone 1D11, 10 μg/ml), or sodium metatungstate (50 μM) are plated onto respective wells of a 96 well round bottom plate. After at least five rounds of gentle aspiration and washing of the 10 cm plate containing the peritoneal lavage cells, adherent peritoneal macrophages are removed with a cell scraper, collected, and counted. 10₅ peritoneal macrophages are plated per indicated well of the 96 well plates. CD8₊ T cells are purified from ACK-lysed splenocytes from C57BL/6 mice utilizing the Mouse CD8a₊ T Cell Isolation Kit and then 10₅ purified CD8₊ T cells are plated onto in T cell medium with 50 IU/ml murine IL-2. Certain wells are incubated with 10₅ anti-mouse CD3/CD28 Dynabeads for T cell activation. Flow cytometry is performed after 48 hours of culture.

参考

https://pubmed.ncbi.nlm.nih.gov/16997558/
https://pubmed.ncbi.nlm.nih.gov/34115989/

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

A2AR-mediated CXCL5 upregulation on macrophages promotes NSCLC progression via NETosis [ Cancer Immunol Immunother, 2024, 73(6):108]	PubMed: 38642131
CD39 pathway inhibits Th1 cell function in tuberculosis [ Immunology, 2022, 10.1111/imm.13493]	PubMed: 35574713
Tim-4+ cavity-resident macrophages impair anti-tumor CD8+ T cell immunity [ Cancer Cell, 2021, 39(7):973-988.e9]	PubMed: 34115989

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。