Sodium Monofluorophosphate

製品コードS4013 バッチS401301

印刷

化学情報

Chemical Structure Synonyms NSC248 Storage
(From the date of receipt)		 3 years -20°C powder
1 years -80°C in solvent
化学式

FNa2O3P
分子量 143.95 CAS No. 10163-15-2
Solubility (25°C)* 体外 Water 29 mg/mL (201.45 mM)
DMSO Insoluble
Ethanol Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明 Sodium Monofluorophosphate (NSC248) is a competitive inhibitor of pyruvate kinase and alkaline phosphatase with Ki of 3.4 mM and 69 μM, respectively, which also irreversibly inhibits phosphorylase phosphatase with Ki of 0.5 mM.
in vitro

Monofluorophosphate (MFP) was an alternative substrate for chick bone ALP. MFP competed for the PNPP binding site on chick bone ALP. The value of Ki for MFP inhibition of chick bone ALP-dependent PNPP hydrolysis was pH dependent. Inhibition of ALP activity by MFP could not be attributed to contaminating F or Pi in the MFP solutions, since Ki values for inhibitions by those compounds were >1 mM and 0.3 mM, at pH 7.4, respectively.[2]
in vivo

Monofluorophosphate displays potent activity on bone immobilization in vivo. Rats supplied with drinking water containing 50 ppm F as Monofluorophosphate show a significant increase in the bone sickness of mobile tibia and immobile tibia, compared with fluoride-free water group (1.48 vs 0.98 mm, 0.79 vs 0.40 mm, respectively). Monofluorophosphate does not change the specific gravities of the immobilized and non-immobilized tibiae of the rats. Administration of Monofluorophosphate increases the mineralization rate of bones and counteracts the decrease in bone thickness, ash content and specific gravity induced by immobilization, without changing the Ca/P ratio of the ash. [3]

プロトコル(参考用のみ)

キナーゼアッセイ Alkaline phosphatase activity assay
ALP activity is measured colorimetrically using PNPP as a substrate in carbonate/tris buffers containing 1 mM MgCl2, in 96-place tissue culture microwell plates (total reaction volume = 0.3 mL). Reactions are initiated by addition of the enzyme, and incubated at ambient room temperature (22 ℃). Production of p-nitrophenolate is monitored by measuring the time-dependent change in absorbance at 410 nm, using a Dynatech automatic microwell plate reader.

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

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