Datasheet

生物学的記述

Specificity	Somatostatin Receptor 1/SSTR1 Antibody [F9M21] detects endogenous levels of total Somatostatin Receptor 1/SSTR1 protein.
Background	Somatostatin Receptor 1 (SSTR1) is a G protein-coupled receptor (GPCR) subtype belonging to the somatostatin receptor family that mediates inhibitory effects of the peptide hormone somatostatin, regulating hormone secretion, cell proliferation, and neurotransmission in the brain, kidney, and lung. SSTR1 features the GPCR hallmark of seven transmembrane α-helices, an extracellular N-terminus, and intracellular C-terminus, with conserved motifs such as the DRY motif in the third intracellular loop critical for signal transduction. Key ligand-binding residues include Q6.55 and S7.34, which form part of the extracellular loops that create the ligand-binding pocket, showing higher affinity for somatostatin-14 (SRIF-14) over SRIF-28. The SSTR1 gene is intronless and located on chromosome 14q13. It couples to pertussis toxin-sensitive Giα proteins to inhibit adenylyl cyclase, reducing intracellular cAMP levels, modulate Ca2+ and K+ channels to hyperpolarize cells, and activate protein tyrosine phosphatases that mediate antiproliferative effects via MAPK pathway modulation. SSTR1 can form homo- and heterodimers (with SSTR5), which influence receptor signaling. Regulation of SSTR1 includes agonist-induced phosphorylation, rapid desensitization, and notably, unlike other SSTRs, limited internalization leading to potential upregulation on the cell surface, affecting therapeutic outcomes. Clinically, SSTR1 is highly expressed in neuroendocrine tumors, acromegaly, and various cancers (pituitary, colorectal, breast, gliomas), where it modulates hormone secretion, tumor growth, and neuronal signaling.

Specificity

Somatostatin Receptor 1/SSTR1 Antibody [F9M21] detects endogenous levels of total Somatostatin Receptor 1/SSTR1 protein.

Background

Somatostatin Receptor 1 (SSTR1) is a G protein-coupled receptor (GPCR) subtype belonging to the somatostatin receptor family that mediates inhibitory effects of the peptide hormone somatostatin, regulating hormone secretion, cell proliferation, and neurotransmission in the brain, kidney, and lung. SSTR1 features the GPCR hallmark of seven transmembrane α-helices, an extracellular N-terminus, and intracellular C-terminus, with conserved motifs such as the DRY motif in the third intracellular loop critical for signal transduction. Key ligand-binding residues include Q6.55 and S7.34, which form part of the extracellular loops that create the ligand-binding pocket, showing higher affinity for somatostatin-14 (SRIF-14) over SRIF-28. The SSTR1 gene is intronless and located on chromosome 14q13. It couples to pertussis toxin-sensitive Giα proteins to inhibit adenylyl cyclase, reducing intracellular cAMP levels, modulate Ca2+ and K+ channels to hyperpolarize cells, and activate protein tyrosine phosphatases that mediate antiproliferative effects via MAPK pathway modulation. SSTR1 can form homo- and heterodimers (with SSTR5), which influence receptor signaling. Regulation of SSTR1 includes agonist-induced phosphorylation, rapid desensitization, and notably, unlike other SSTRs, limited internalization leading to potential upregulation on the cell surface, affecting therapeutic outcomes. Clinically, SSTR1 is highly expressed in neuroendocrine tumors, acromegaly, and various cancers (pituitary, colorectal, breast, gliomas), where it modulates hormone secretion, tumor growth, and neuronal signaling.

使用情報

Application

WB, IF

Dilution

WB	IF
1:500	1:250 - 1:500

Reactivity

Human

Source

Rabbit Monoclonal Antibody

MW

43 kDa

Storage Buffer

PBS, pH 7.2+50% Glycerol+0.05% BSA+0.01% NaN3

Storage
(from the date of receipt)

-20°C (avoid freeze-thaw cycles), 2 years

プロトコール

References

https://pubmed.ncbi.nlm.nih.gov/32875251/
https://pubmed.ncbi.nlm.nih.gov/39361640/

Application Data

WB

Validated by Selleck

Lane 1: Human fetal lung, Lane 2: Human small intestine