SP-8356

製品コードE0487 バッチE048701

印刷

化学情報

Synonyms

Storage
(From the date of receipt)

3 years -20°C powder

化学式

C₁₈H₂₀O₄

分子量

300.35

CAS No.

1454885-45-0

Solubility (25°C)*

体外

DMSO

60 mg/mL (199.76 mM)

Ethanol

8 mg/mL (26.63 mM)

Water

Insoluble

体内（毎回新しく調製した物を用意してください）

Clear solution

5%DMSO 1 Corn oil

0.75mg/ml

Taking the 1 mL working solution as an example, add 50 μL of 15 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

溶剤液（一定の濃度）を調合する

生物活性

製品説明	SP-8356, an anti-inflammatory synthetic verbenone derivative, is a CD147 inhibitor with respect to its regulation of breast cancer cell behavior and cancer progression.
in vitro	SP-8356, a synthetic anti-inflammatory verbenone derivative, exerts remarkable anti-atherosclerotic effects through inhibiting CD147-CypA interactions and reducing CypA-induced MMP-9 activation and monocyte adhesion.^[2]
in vivo	SP-8356 suppresses collagenase activity, MMP-9, and CypA, preventing the formation of plaque and attenuates its vulnerability in mice models of atherosclerosis.^[2]

プロトコル(参考用のみ)

キナーゼアッセイ
キナーゼアッセイ
細胞アッセイ	細胞株	mouse brain endothelial cell line (bEnd.3); mouse macrophage cell line (RAW 264.7) cells
	濃度	0.01, 0.1, 1, 10 µM
	反応時間
	実験の流れ	The bEnd. 3 cells are transfected with individual plasmids using Lipofectamine 3000 for 48 h. To release HA-tagged CypA into extracellular medium, transfected cells are exposed to oxygen-glucose deprivation (OGD) for 6 h and the culture supernatants collected. RAW 264.7 cells are co-treated with the collected supernatant fractions and different concentrations of SP-8356 for 30 min at 37 ℃, harvested and lysed with lysis buffer containing a protease inhibitor cocktail for 60 min on ice. Lysates are centrifuged at 15,000 rpm for 15 min at 4 ℃ and the supernatant fractions incubated with monoclonal anti-CD147 antibody at 4 ℃ overnight. Next, supernatants are added to pre-washed protein A Sepharose beads and incubated for 2 h at 4 ℃. Beads are rewashed and bound proteins eluted by boiling in sodium dodecyl sulfate (SDS) sample buffer, followed by separation via 15% SDS-polyacrylamide gel electrophoresis (PAGE). To examine protein expression, clarified lysates are electrophoresed, transferred onto nitrocellulose membrane, probed with anti-HA and anti-CD147 and detected using an electrochemiluminescence (ECL) assay kit.
動物実験	動物モデル	ApoE KO mice
	投薬量	50 mg/kg
	投与方法	p.o.

参考

Selleckの高級品が、幾つかの出版された研究調査結果（以下を含む）で使われた：

Myricetin, a natural inhibitor of CD147, increases sensitivity of cisplatin in ovarian cancer [ Expert Opin Ther Targets, 2024, 1-13.]	PubMed: 38235574

Myricetin, a natural inhibitor of CD147, increases sensitivity of cisplatin in ovarian cancer [ Expert Opin Ther Targets, 2024, 1-13.]

PubMed: 38235574

長期の保管のために-20°Cの下で製品を保ってください。

人間や獣医の診断であるか治療的な使用のためにでない。

各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。