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Synonyms | N/A | Storage (From the date of receipt) |
3 years -20°C powder | |
化学式 | C22H16N2O |
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分子量 | 324.38 | CAS No. | 1253491-42-7 | |
Solubility (25°C)* | 体外 | DMSO | 65 mg/mL (200.38 mM) | |
Ethanol | 65 mg/mL (200.38 mM) | |||
Water | Insoluble | |||
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. |
製品説明 | SP141 is a new class of MDM2 inhibitor that promotes MDM2 auto-ubiquitination and degradation, and reduces levels of MDM2 in pancreatic cancer cell lines, as well as their proliferation and ability to form tumors in nude mice. SP141 inhibits human pancreatic cancer cell growth with IC50 values of less than 0.5 μM (0.38–0.50 μM) in a p53-independent manner. |
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in vitro | SP141 can bound directly to MDM2, promoting its auto-ubiquitination and degradation by the proteasome. SP141 reduces levels of MDM2 in pancreatic cancer cell lines, as well as their proliferation, with 50% inhibitory concentrations <0.5 μM (0.38–0.50 μM). Increasing concentrations of SP141 induces increasing levels of apoptosis and G2–M phase arrest of pancreatic cancer cell lines, whether or not they expressed functional P53.[1] |
in vivo | SP-141 (40 mg/kg; administered by i.p. injection; 5 d/wk for about three weeks) suppresses pancreatic tumor growth in both xenograft and orthotopic mouse models.[1] |
細胞アッセイ | 細胞株 | Humen pancreatic cancer cell lines |
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濃度 | 0.01-10 μM | |
反応時間 | 72 h | |
実験の流れ | Human pancreatic cancer cell lines (HPAC, Panc-1, AsPC-1, and Mia-Paca-2) are treated with various concentrations of SP141 or vehicle controls. And then cells are used to determine the effects of SP141 on the cell viability (MTT assay), colony formation,39 proliferation (BrdUrd incorporation assay), apoptosis, and cell cycle distribution. |
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動物実験 | 動物モデル | Nude mice bearing Panc-1 xenograft tumors |
投薬量 | 40 mg/kg | |
投与方法 | i.p. |
長期の保管のために-20°Cの下で製品を保ってください。
人間や獣医の診断であるか治療的な使用のためにでない。
各々の製品のための特定の保管と取扱い情報は、製品データシートの上で示されます。大部分のSelleck製品は、推薦された状況の下で安定です。製品は、推薦された保管温度と異なる温度で、時々出荷されます。長期の保管のために必要とされてそれと異なる温度で、多くの製品は、短期もので安定です。品質を維持するが、夜通しの積荷のために最も経済的な貯蔵状況を用いてあなたの送料を保存する状況の下に、製品が出荷されることを、我々は確実とします。製品の受領と同時に、製品データシートの上で貯蔵推薦に従ってください。